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The potential role ...
The potential role of collagen type VII in breast cancer proliferation
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- Pérez-Díaz, Sergio (författare)
- Umeå universitet,Institutionen för medicinsk och translationell biologi,Department of Laboratory Medicine, Division of Clinical Physiology, Karolinska Institute, Stockholm, Sweden
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- Lindberg, Jessica (författare)
- Umeå universitet,Institutionen för medicinsk och translationell biologi,Institutionen för diagnostik och intervention,Kirurgi
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- Anerillas, Luis Oliveros, 1995- (författare)
- Umeå universitet,Institutionen för medicinsk och translationell biologi
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- Kingham, Paul J. (författare)
- Umeå universitet,Institutionen för medicinsk och translationell biologi
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- Sund, Malin (författare)
- Umeå universitet,Institutionen för diagnostik och intervention,Kirurgi,Department of Surgery/CLINICUM, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
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- Rask, Gunilla (författare)
- Umeå universitet,Institutionen för diagnostik och intervention,Kirurgi
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- Svensson, Johan, 1978- (författare)
- Umeå universitet,Statistik
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- Jansson, Malin, 1978- (författare)
- Umeå universitet,Institutionen för diagnostik och intervention,Kirurgi
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- Wiberg, Rebecca, 1988- (författare)
- Umeå universitet,Institutionen för medicinsk och translationell biologi,Institutionen för diagnostik och intervention,Handkirurgi
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(creator_code:org_t)
- Springer Nature, 2024
- 2024
- Engelska.
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Ingår i: Cancer Cell International. - : Springer Nature. - 1475-2867. ; 24:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Breast cancer is the most common cancer in women. Cancer cells can persist in a prolonged dormant state for years without any clinical evidence of disease creating an urgent need to better understand the molecular mechanisms leading to relapse. This study aimed to identify extracellular matrix (ECM) components associated with hypoxia-induced breast cancer dormancy. The effects of selected ECM proteins on breast cancer cell proliferation were analyzed, along with their correlation with established prognostic markers in human breast cancer tissue.Materials and methods: Screening of extracellular matrix proteins was performed in hypoxia-induced dormant MCF-7 breast cancer cells. Proliferation of MCF-7 cells in vitro was subsequently determined in the presence of recombinant ColVII. Adipose tissue-derived mesenchymal stem cells (AdMSCs) subpopulation overexpressing ColVII were indirectly isolated by ColVII receptor integrin-α6 specific antibodies. AdMSCs- MCF-7 3D spheroid cultures were generated to model solid tumour conditions. In addition, the association between ColVII and various prognostic markers was evaluated in clinical samples of human breast cancer tissue.Results: Dormant MCF-7 cells showed an elevated expression of ColVII while MCF-7 cells cultured on ColVII exhibited reduced proliferation in vitro. In AdMSCs-MCF-7 3D spheroids, a reduced proliferation of MCF-7 cells was observed in Int-α6+/ ColVIIhigh compared with Int-α6-/ ColVIIlow AdMSCs spheroids. In human tissue, high ColVII expression correlated to several positive prognostic markers. Staining for Cytokeratin-5 revealed that ColVIIhigh-expressing cells were predominantly myoepithelial cells.Conclusion: ColVII is associated with reduced proliferation of breast cancer cells in vitro. ColVII is strongly expressed in myoepithelial cells and in breast cancer tissue the high ColVII expression correlates with several well-known positive prognostic markers, highlighting its potential as a prognostic marker in breast cancer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Breast cancer
- Collagen type VII
- Extracellular matrix
- Mesenchymal stem cell
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Pérez-Díaz, Serg ...
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Lindberg, Jessic ...
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Anerillas, Luis ...
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Kingham, Paul J.
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Sund, Malin
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Rask, Gunilla
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Svensson, Johan, ...
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Jansson, Malin, ...
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Wiberg, Rebecca, ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Cancer Cell Inte ...
- Av lärosätet
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Umeå universitet
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Karolinska Institutet