Sökning: onr:"swepub:oai:DiVA.org:umu-2568" >
Identification of p...
Identification of plant glutaredoxin targets
-
Rouhier, Nicolas (författare)
-
Gelhaye, Eric (författare)
-
Dietz, Karl Josef (författare)
-
visa fler...
-
Jacquot, Jean-Pierre (författare)
-
- Wingsle, Gunnar (författare)
- Umeå universitet,Umeå Plant Science Centre (UPSC)
-
Villarejo, Arsenio (författare)
-
Srivastava, Manoj (författare)
-
- Keech, Olivier (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
-
Droux, Michel (författare)
-
Finkemeier, Iris (författare)
-
- Samuelsson, Göran, 1951- (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
-
visa färre...
-
(creator_code:org_t)
- Larchmont, NY : Mary Ann Liebert, 2005
- 2005
- Engelska.
-
Ingår i: Antioxidants and Redox Signaling. - Larchmont, NY : Mary Ann Liebert. - 1523-0864 .- 1557-7716. ; 7:7-8, s. 919-929
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Glutaredoxins (Grxs) are small ubiquitous proteins of the thioredoxin (Trx) family, which catalyze dithiol–disulfide exchange reactions or reduce protein-mixed glutathione disulfides. In plants, several Trx-interacting proteins have been isolated from different compartments, whereas very few Grx-interacting proteins are known. We describe here the determination of Grx target proteins using a mutated poplar Grx, various tissular and subcellular plant extracts, and liquid chromatography coupled to tandem mass spectrometry detection. We have identified 94 putative targets, involved in many processes, including oxidative stress response [peroxiredoxins (Prxs), ascorbate peroxidase, catalase], nitrogen, sulfur, and carbon metabolisms (methionine synthase, alanine aminotransferase, phosphoglycerate kinase), translation (elongation factors E and Tu), or protein folding (heat shock protein 70). Some of these proteins were previously found to interact with Trx or to be glutathiolated in other organisms, but others could be more specific partners of Grx. To substantiate further these data, Grx was shown to support catalysis of the stroma β-type carbonic anhydrase and Prx IIF of Arabidopsis thaliana, but not of poplar 2-Cys Prx. Overall, these data suggest that the interaction could occur randomly either with exposed cysteinyl disulfide bonds formed within or between target proteins or with mixed disulfides between a protein thiol and glutathione.
Nyckelord
- Adenosine Triphosphate/metabolism
- Arabidopsis/enzymology/*metabolism
- Carbon Dioxide/metabolism
- Cell Respiration/radiation effects
- Citric Acid/metabolism
- Electron Transport
- Molecular Chaperones/metabolism
- Oxidative Stress
- Oxidoreductases/*metabolism
- Oxygen/metabolism
- Peroxidases/metabolism
- Photochemistry
- Polysaccharides/biosynthesis/metabolism
- Protein Biosynthesis
- Sulfur/metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Rouhier, Nicolas
-
Gelhaye, Eric
-
Dietz, Karl Jose ...
-
Jacquot, Jean-Pi ...
-
Wingsle, Gunnar
-
Villarejo, Arsen ...
-
visa fler...
-
Srivastava, Mano ...
-
Keech, Olivier
-
Droux, Michel
-
Finkemeier, Iris
-
Samuelsson, Göra ...
-
visa färre...
- Artiklar i publikationen
-
Antioxidants and ...
- Av lärosätet
-
Umeå universitet