Sökning: onr:"swepub:oai:DiVA.org:umu-30615" >
Apoptotic signallin...
-
Eriksson, DavidUmeå universitet,Immunologi/immunkemi,Stigbrand
(författare)
Apoptotic signalling in HeLa Hep2 cells following 5 Gy of cobalt-60 gamma radiation
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:umu-30615
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-30615URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
BACKGROUND: The apoptotic signalling pathways involved in the delayed type of apoptosis occurring in HeLa Hep2 cells following radiation were investigated. MATERIALS AND METHODS: HeLa Hep2 cells were exposed to 5 Gy of cobalt-60 radiation. The activation of caspase-2, caspase-8, caspase-9 and effector caspase-3 was investigated by caspase assay plates and Western blots. Cleavage of poly (ADP-ribose) polymerase (PARP) was analysed on Western blots. HeLa Hep2 cells were irradiated with or without preincubation with inhibitors of protein synthesis (cycloheximide, CHX) and caspases, followed by TUNEL staining and caspase assay plate evaluation. RESULTS: Initiator caspases-2, -8, -9, and effector caspase-3, were found to be activated and PARP cleaved following irradiation. CHX completely inhibited the caspase activation and the associated apoptosis. Pretreatment with caspase-2 inhibitor indicated that caspase-2 was involved in the execution of the apoptosis. CONCLUSION: Activation of the apoptotic signalling pathways following irradiation of HeLa Hep2 cells includes components from the intrinsic as well as the extrinsic pathways and seems to require de novo protein synthesis.
Ämnesord och genrebeteckningar
-
Radiation
-
apoptosis
-
caspase-2
-
caspase-8
-
caspase-9
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Löfroth, Per-OlovUmeå universitet,Radiofysik
(författare)
-
Johansson, LennartUmeå universitet,Radiofysik(Swepub:umu)lejo0023
(författare)
-
Riklund, KatrineUmeå universitet,Diagnostisk radiologi(Swepub:umu)kaah0001
(författare)
-
Stigbrand, TorgnyUmeå universitet,Immunologi/immunkemi,Stigbrand(Swepub:umu)tost0001
(författare)
-
Umeå universitetImmunologi/immunkemi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Anticancer Research29:11, s. 4361-43660250-70051791-7530
Internetlänk
Hitta via bibliotek
Till lärosätets databas