Sökning: onr:"swepub:oai:DiVA.org:umu-45876" >
Expression and secr...
Expression and secretion of the novel adipokine tartrate-resistant acid phosphatase from adipose tissues of obese and lean women
-
- Lång, P (författare)
- Karolinska Institutet
-
- Zakaroff-Girard, A (författare)
- Karolinska Institutet
-
- Wåhlén, K (författare)
- Karolinska Institutet
-
visa fler...
-
- Andersson, Jonas (författare)
- Umeå universitet,Medicin
-
- Olsson, Tommy (författare)
- Umeå universitet,Medicin
-
Bambace, C (författare)
-
Jocken, J (författare)
-
Bouloumié, A (författare)
-
Andersson, G (författare)
-
- Arner, P (författare)
- Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- 2011-03-08
- 2011
- Engelska.
-
Ingår i: International Journal of Obesity. - : Springer Science and Business Media LLC. - 0307-0565 .- 1476-5497. ; 35:12, s. 1502-1510
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Objective: Tartrate-resistant acid phosphatase (TRAP) expressed by adipose tissue macrophages (ATMs) induces mice obesity and human adipocyte differentiation in vitro. This study aimed to investigate whether TRAP was secreted differently from human obese versus lean adipose tissues and to identify the cellular source of adipose tissue TRAP.Design: Subcutaneous adipose tissues obtained from healthy subjects. Enzyme-linked immunosorbent assays (ELISAs) for total (5a+5b) and cleaved TRAP (5b) were used. TRAP secretion was determined in adipose tissue biopsies, and mRNA expression was studied in cell types isolated from the same.Subjects: Results of 24 lean and 24 obese women (in vitro) and 8 subjects (in vivo) were compared. The main outcome measurements were TRAP expression and secretion in vitro and in vivo.Results: In-house total TRAP ELISA showed high sensitivity and a coefficient of variance of 11%. Adipose secretion of total TRAP was linear in vitro with time and was evident in vivo. Total TRAP secretion in vitro was similar in lean and obese women expressed per unit weight of the adipose tissue but correlated positively with the number/size of adipocytes (P≤0.01) and with adipose secretion of tumor necrosis factor-α and interleukin-6 (P<0.01). TRAP 5b was not secreted from the adipose tissue. ATMs displayed highest cellular expression of TRAP mRNA in adipose tissue cells derived from lean or obese women.Conclusions: TRAP is a novel human adipokine produced by macrophages and secreted from the subcutaneous adipose tissue in vivo and in vitro. Secretion is linked to the size and number of adipocytes, as well as to concomitant secretion of inflammatory mediators, suggesting that TRAP is involved in fat accumulation and adipose inflammation. International Journal of Obesity advance online publication, 8 March 2011; doi:10.1038/ijo.2011.17.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- tartrate-resistant acid phosphatase
- acid phosphatase type 5
- macrophages
- adipose tissue
- adipokine
- inflammation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Lång, P
-
Zakaroff-Girard, ...
-
Wåhlén, K
-
Andersson, Jonas
-
Olsson, Tommy
-
Bambace, C
-
visa fler...
-
Jocken, J
-
Bouloumié, A
-
Andersson, G
-
Arner, P
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Endokrinologi oc ...
- Artiklar i publikationen
-
International Jo ...
- Av lärosätet
-
Umeå universitet
-
Karolinska Institutet