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Signatures of T cel...
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Eneslätt, KjellUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)
(författare)
Signatures of T cells as correlates of immunity to Francisella tularensis
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-03-06
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Public Library of Science (PLoS),2012
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:umu-55370
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-55370URI
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https://doi.org/10.1371/journal.pone.0032367DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Tularemia or vaccination with the live vaccine strain (LVS) of Francisella tularensis confers long-lived cell-mediated immunity. We hypothesized that this immunity depends on polyfunctional memory T cells, i.e., CD4(+) and/or CD8(+) T cells with the capability to simultaneously express several functional markers. Multiparametric flow cytometry, measurement of secreted cytokines, and analysis of lymphocyte proliferation were used to characterize in vitro recall responses of peripheral blood mononuclear cells (PBMC) to killed F. tularensis antigens from the LVS or Schu S4 strains. PBMC responses were compared between individuals who had contracted tularemia, had been vaccinated, or had not been exposed to F. tularensis (naive). Significant differences were detected between either of the immune donor groups and naive individuals for secreted levels of IL-5, IL-6, IL-10, IL-12, IL-13, IFN-gamma, MCP-1, and MIP-1 beta. Expression of IFN-gamma, MIP-1 beta, and CD107a by CD4(+)CD45RO(+) or CD8(+) CD45RO(+) T cells correlated to antigen concentrations. In particular, IFN-gamma and MIP-1 beta strongly discriminated between immune and naive individuals. Only one cytokine, IL-6, discriminated between the two groups of immune individuals. Notably, IL-2- or TNF-alpha-secretion was low. Our results identify functional signatures of T cells that may serve as correlates of immunity and protection against F. tularensis.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Normark, MonicaUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)(Swepub:umu)moaann01
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Björk, RafaelUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)(Swepub:umu)ralbjk02
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Rietz, CeciliaUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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Zingmark, CarlUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)(Swepub:umu)cazi0001
(författare)
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Wolfraim, Lawrence A
(författare)
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Stöven, SvenjaUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS)(Swepub:umu)svst0001
(författare)
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Sjöstedt, AndersUmeå universitet,Klinisk bakteriologi,Molekylär Infektionsmedicin, Sverige (MIMS),Arcum(Swepub:umu)ansj0004
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Umeå universitetKlinisk bakteriologi
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Ingår i:PLOS ONE: Public Library of Science (PLoS)7:3, s. e32367-1932-6203
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