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Evidence for IFN-be...
Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.
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Borg, H (författare)
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Nordensson, I (författare)
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- Ny, Tor (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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visa fler...
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Lundgren, E (författare)
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visa färre...
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(creator_code:org_t)
- 1985
- 1985
- Engelska.
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Ingår i: Applied Biochemistry and Biotechnology. - 0273-2289 .- 1559-0291. ; 11:2, s. 111-22
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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