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Cloning and charact...
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Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik
(författare)
Cloning and characterization of a cDNA for rat tissue-type plasminogen activator.
- Artikel/kapitelEngelska1988
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LIBRIS-ID:oai:DiVA.org:umu-59886
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59886URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Two partly overlapping lambda gt11 cDNA clones coding for the 22S rat tissue-type plasminogen activator (t-PA) mRNA were isolated. The cDNA sequences cover 2445 nucleotides of the mRNA, including a 5' untranslated region of 31 nucleotides, an open reading frame of 1677 nucleotides, a 3' untranslated region of 737 nucleotides, and a poly(A) tail. The open reading frame codes for a 17-amino-acid signal peptide, a propeptide with 12 amino acids, and the mature protein with 530 amino acids. Rat t-PA has 81% and 92% amino acid sequence identity with the human and mouse counterparts and an equal distribution of conserved amino acids, suggesting that the proteins can fold into identical three-dimensional structures. The rat t-PA sequence contains two putative N-glycosylation sites at Asn-120 and Asn-452, while human t-PA has an additional glycosylation site at Asn-187. The site at Asn-187 is glycosylated in the human protein, revealing a different glycosylation pattern between the human and rat proteins.
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Leonardsson, G
(författare)
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Hsueh, A J
(författare)
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Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:DNA (Mary Ann Liebert, Inc.)7:10, s. 671-70198-0238
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