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Sökning: onr:"swepub:oai:DiVA.org:umu-67883" > Over-expression of ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003175naa a2200349 4500
001oai:DiVA.org:umu-67883
003SwePub
008130406s2003 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-678832 URI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Melgar, S4 aut
2451 0a Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis.
264 1c 2003
338 a print2 rdacarrier
520 a Ulcerative colitis (UC), a chronic inflammatory bowel disease, exhibits pronounced increase of T lymphocytes in the inflamed mucosa. To understand the role of intestinal T lymphocytes in the pathogenesis of UC their cytokine production in the mucosa was analysed. Intestinal T lymphocytes of UC, Crohn's disease and control patients were analysed for cytokine mRNA levels by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) directly after isolation without in vitro stimulation. Frequencies of cytokine positive cells were determined in UC and control colon by immunomorphometry. T lymphocytes in normal colon expressed interleukin (IL)-2, interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta1, but not IL-4, IL-5 or IL-10. In UC, a highly significant increase in IL-10 mRNA levels in T lymphocytes and an increased frequency of IL-10 positive cells was seen in colon. IL-10 mRNA levels were also elevated in T lymphocytes of the non-inflamed ileum and correlated with disease activity at both locations. CD4+ T lymphocytes were the major source of IL-10 mRNA. IL-2, IFN-gamma and TNF-alpha mRNA levels were decreased in colonic T lymphocytes, and virtually no IL-2, IFN-gamma, TNF-alpha or TGF-beta positive cells were detected in basal lymphoid aggregates. However, scattered IL-10 positive cells were found here. Lamina propria outside the aggregates contained IL-10-, IFN-gamma, TNF-alpha and TGF-beta but not IL-2 positive cells. T cells of UC patients did not express IL-4 or IL-5. Taken, together the data suggest a generalized activation of IL-10 producing CD4+ T cells along the intestine of UC patients. The local environment seems to determine the biological consequences of elevated IL-10.
650 7a NATURVETENSKAPx Biologix Immunologi0 (SwePub)106052 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng
7001 a Yeung, M M-W4 aut
7001 a Bas, A4 aut
7001 a Forsberg, G4 aut
7001 a Suhr, O4 aut
7001 a Oberg, A4 aut
7001 a Hammarström, Stenu Umeå universitet,Immunologi/immunkemi4 aut0 (Swepub:umu)stha0002
7001 a Danielsson, A4 aut
7001 a Hammarström, Marie-Louiseu Umeå universitet,Immunologi/immunkemi4 aut0 (Swepub:umu)maha0008
7102 a Umeå universitetb Immunologi/immunkemi4 org
7730 t Clinical and Experimental Immunologyg 134:1, s. 127-37q 134:1<127-37x 0009-9104x 1365-2249
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-67883

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