Analysis of plant growth regulating substances

• Natural plant growth regulators (phytohormones) are a group of organic compounds which, in very small amounts, act as regulators of physiological processes in plants.Methods were developed for the analysis of phytohormones in samples from Norway spruce (Picea abies (L.) Karst.) and Scots pine (Pinus sylvestris (L.) Karst»). Identification of abscisic acid, 3-indoleacetic acid, gibbe-rellin Ag and the conjugate N-(3-indoleacetyl)aspartic acid was performed by GC-MS as their methyl esters. A quantitative determination of abscisic acid was made by GC-ECD and this method was also applied to anther samples of Anemone canadensis. 3-Indole-acetic acid and N-(3-indoleacetyl)aspartic acid were quantified by reversed-phase HPLC and spectrofluorimetric detection. Dichlorophene, used as a growth regulator in containerized seedlings of pine and spruce, was analysed by GC-MID in peat and paper.

Ämnesord

NATURVETENSKAP  -- Kemi -- Organisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Organic Chemistry (hsv//eng)

Nyckelord

Plant regulators

phytohormones

pine

spruce

anther

abscisic acid

3-indoleacetic acid

gibberellin Ag

N-(3-indoleacetyl)-aspartic acid

dichlorophene

Amberlite XAD-7

identification

quantification

GC

HPLC

GC-MS

GC-MID

Hormoner

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vet (ämneskategori)

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