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Vibrio cholerae eva...
Vibrio cholerae evades neutrophil extracellular traps by the activity of two extracellular nucleases
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- Seper, Andrea (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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- Hosseinzadeh, Ava (författare)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Gorkiewicz, Gregor (författare)
- University of Graz, Graz, Austria
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- Lichtenegger, Sabine (författare)
- University of Graz, Graz, Austria
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- Roier, Sandro (författare)
- University of Graz, Graz, Austria
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- Leitner, Deborah R (författare)
- University of Graz, Graz, Austria
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- Röhm, Marc (författare)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för klinisk mikrobiologi
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- Grutsch, Andreas (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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- Reidl, Joachim (författare)
- University of Graz, Graz, Austria
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- Urban, Constantin F (författare)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för klinisk mikrobiologi
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- Schild, Stefan (författare)
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
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(creator_code:org_t)
- 2013-09-05
- 2013
- Engelska.
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Ingår i: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7366 .- 1553-7374. ; 9:9
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- The Gram negative bacterium Vibrio cholerae is the causative agent of the secretory diarrheal disease cholera, which has traditionally been classified as a noninflammatory disease. However, several recent reports suggest that a V. cholerae infection induces an inflammatory response in the gastrointestinal tract indicated by recruitment of innate immune cells and increase of inflammatory cytokines. In this study, we describe a colonization defect of a double extracellular nuclease V. cholerae mutant in immunocompetent mice, which is not evident in neutropenic mice. Intrigued by this observation, we investigated the impact of neutrophils, as a central part of the innate immune system, on the pathogen V. cholerae in more detail. Our results demonstrate that V. cholerae induces formation of neutrophil extracellular traps (NETs) upon contact with neutrophils, while V. cholerae in return induces the two extracellular nucleases upon presence of NETs. We show that the V. cholerae wild type rapidly degrades the DNA component of the NETs by the combined activity of the two extracellular nucleases Dns and Xds. In contrast, NETs exhibit prolonged stability in presence of the double nuclease mutant. Finally, we demonstrate that Dns and Xds mediate evasion of V. cholerae from NETs and lower the susceptibility for extracellular killing in the presence of NETs. This report provides a first comprehensive characterization of the interplay between neutrophils and V. cholerae along with new evidence that the innate immune response impacts the colonization of V. cholerae in vivo. A limitation of this study is an inability for technical and physiological reasons to visualize intact NETs in the intestinal lumen of infected mice, but we can hypothesize that extracellular nuclease production by V. cholerae may enhance survival fitness of the pathogen through NET degradation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Seper, Andrea
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Hosseinzadeh, Av ...
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Gorkiewicz, Greg ...
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Lichtenegger, Sa ...
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Roier, Sandro
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Leitner, Deborah ...
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visa fler...
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Röhm, Marc
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Grutsch, Andreas
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Reidl, Joachim
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Urban, Constanti ...
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Schild, Stefan
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Mikrobiologi ino ...
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PLoS Pathogens
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Umeå universitet