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Efficiency of photo...
Efficiency of photosynthetic water oxidation at ambient and depleted levels of inorganic carbon
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- Shevela, Dmitriy (författare)
- Umeå universitet,Kemiska institutionen,Max Planck Institute for Chemical Energy Conversion, Mülheim, Germany,Umea Univ, Chem Biol Ctr, Dept Chem, S-90187 Umea, Sweden.;Max Planck Inst Chem Energy Convers, D-45470 Mulheim, Germany.;Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, S-90187 Umea, Sweden.
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- Nöring, Birgit (författare)
- Max Planck Institute for Chemical Energy Conversion, Mülheim, Germany,Max Planck Inst Chem Energy Convers, D-45470 Mulheim, Germany.
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- Koroidov, Sergey (författare)
- Umeå universitet,Kemiska institutionen,Umea Univ, Chem Biol Ctr, Dept Chem, S-90187 Umea, Sweden.
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- Shutova, Tatyana (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Samuelsson, Göran, 1951- (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, S-90187 Umea, Sweden.
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- Messinger, Johannes (författare)
- Umeå universitet,Kemiska institutionen,Max Planck Institute for Chemical Energy Conversion, Mülheim, Germany,Umea Univ, Chem Biol Ctr, Dept Chem, S-90187 Umea, Sweden.;Max Planck Inst Chem Energy Convers, D-45470 Mulheim, Germany.
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(creator_code:org_t)
- 2013-07-05
- 2013
- Engelska.
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Ingår i: Photosynthesis Research. - : Springer. - 0166-8595 .- 1573-5079. ; 117:1-3, s. 401-412
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Over 40 years ago, Joliot et al. (Photochem Photobiol 10:309-329, 1969) designed and employed an elegant and highly sensitive electrochemical technique capable of measuring O2 evolved by photosystem II (PSII) in response to trains of single turn-over light flashes. The measurement and analysis of flash-induced oxygen evolution patterns (FIOPs) has since proven to be a powerful method for probing the turnover efficiency of PSII. Stemler et al. (Proc Natl Acad Sci USA 71(12):4679-4683, 1974), in Govindjee's lab, were the first to study the effect of "bicarbonate" on FIOPs by adding the competitive inhibitor acetate. Here, we extend this earlier work by performing FIOPs experiments at various, strictly controlled inorganic carbon (Ci) levels without addition of any inhibitors. For this, we placed a Joliot-type bare platinum electrode inside a N2-filled glove-box (containing 10-20 ppm CO2) and reduced the Ci concentration simply by washing the samples in Ci-depleted media. FIOPs of spinach thylakoids were recorded either at 20-times reduced levels of Ci or at ambient Ci conditions (390 ppm CO2). Numerical analysis of the FIOPs within an extended Kok model reveals that under Ci-depleted conditions the miss probability is discernibly larger (by 2-3 %) than at ambient conditions, and that the addition of 5 mM HCO3 (-) to the Ci-depleted thylakoids largely restores the original miss parameter. Since a "mild" Ci-depletion procedure was employed, we discuss our data with respect to a possible function of free or weakly bound HCO3 (-) at the water-splitting side of PSII.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Nyckelord
- Flash-induced oxygen evolution patterns
- S states
- An extended Kok model
- Hydrogen carbonate (bicarbonate)
- Photosynthetic water oxidation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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