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Two-photon excitati...
Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments
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Habenicht, Anja (författare)
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Hjelm, Johan (författare)
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Mukhtar, Emad (författare)
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- Bergström, Fredrik (författare)
- Umeå universitet,Kemiska institutionen
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- Johansson, Lennart B-Å (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- 2002
- 2002
- Engelska.
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Ingår i: Chemical Physics Letters. ; 354:5-6, s. 367-75
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.
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