Sökning: onr:"swepub:oai:DiVA.org:umu-88287" >
Increased paired bo...
Increased paired box transcription factor 8 has a survival function in Glioma
-
Hung, Noelyn (författare)
-
Chen, Yu-Jen (författare)
-
Taha, Ahmad (författare)
-
visa fler...
-
- Olivecrona, Magnus (författare)
- Umeå universitet,Klinisk neurovetenskap
-
Boet, Ronald (författare)
-
Wiles, Anna (författare)
-
Warr, Tracy (författare)
-
Shaw, Alisha (författare)
-
Eiholzer, Ramona (författare)
-
Baguley, Bruce C. (författare)
-
Eccles, Michael R. (författare)
-
Braithwaite, Antony W. (författare)
-
MacFarlane, Martin (författare)
-
Royds, Janice A. (författare)
-
Slatter, Tania (författare)
-
visa färre...
-
(creator_code:org_t)
- 2014-03-06
- 2014
- Engelska.
-
Ingår i: BMC Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 14, s. 159-
- Relaterad länk:
-
https://umu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://bmccancer.bi...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background:The molecular basis to overcome therapeutic resistance to treat glioblastoma remains unclear. The anti-apoptotic b cell lymphoma 2 (BCL2) gene is associated with treatment resistance, and is transactivated by the paired box transcription factor 8 (PAX8). In earlier studies, we demonstrated that increased PAX8 expression in glioma cell lines was associated with the expression of telomerase. In this current study, we more extensively explored a role for PAX8 in gliomagenesis.Methods:PAX8 expression was measured in 156 gliomas including telomerase-negative tumours, those with the alternative lengthening of telomeres (ALT) mechanism or with a non-defined telomere maintenance mechanism (NDTMM), using immunohistochemistry and quantitative PCR. We also tested the affect of PAX8 knockdown using siRNA in cell lines on cell survival and BCL2 expression.Results:Seventy-two percent of glioblastomas were PAX8-positive (80% telomerase, 73% NDTMM, and 44% ALT). The majority of the low-grade gliomas and normal brain cells were PAX8-negative. The suppression of PAX8 was associated with a reduction in both cell growth and BCL2, suggesting that a reduction in PAX8 expression would sensitise tumours to cell death.Conclusions:PAX8 is increased in the majority of glioblastomas and promoted cell survival. Because PAX8 is absent in normal brain tissue, it may be a promising therapeutic target pathway for treating aggressive gliomas.
Nyckelord
- PAX8
- Glioblastoma
- Glioma
- Telomere maintenance mechanism
- Telomerase
- ALT
- BCL2
- Cell survival
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Hung, Noelyn
-
Chen, Yu-Jen
-
Taha, Ahmad
-
Olivecrona, Magn ...
-
Boet, Ronald
-
Wiles, Anna
-
visa fler...
-
Warr, Tracy
-
Shaw, Alisha
-
Eiholzer, Ramona
-
Baguley, Bruce C ...
-
Eccles, Michael ...
-
Braithwaite, Ant ...
-
MacFarlane, Mart ...
-
Royds, Janice A.
-
Slatter, Tania
-
visa färre...
- Artiklar i publikationen
-
BMC Cancer
- Av lärosätet
-
Umeå universitet