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Study of the protei...
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Bárcena-Uribarri, Iván
(författare)
Study of the protein complex, pore diameter, and pore-forming activity of the Borrelia burgdorferi P13 porin
- Artikel/kapitelEngelska2014
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American Society for Biochemistry and Molecular Biology,2014
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:umu-91837
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-91837URI
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https://doi.org/10.1074/jbc.M113.539528DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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P13 is one of the major outer membrane proteins of Borrelia burgdorferi. Previous studies described P13 as a porin. In the present study some structure and function aspects of P13 were studied. P13 showed according to lipid bilayer studies a channel-forming activity of 0.6 nanosiemens in 1 M KCl. Single channel and selectivity measurements demonstrated that P13 had no preference for either cations or anions and showed no voltage-gating up to +/-100 mV. Blue native polyacrylamide gel electrophoresis was used to isolate and characterize the P13 protein complex in its native state. The complex had a high molecular mass of about 300 kDa and was only composed of P13 monomers. The channel size was investigated using non-electrolytes revealing an apparent diameter of about 1.4 nm with a 400-Da molecular mass cut-off. Multichannel titrations with different substrates reinforced the idea that P13 forms a general diffusion channel. The identity of P13 within the complex was confirmed by second dimension SDS-PAGE, Western blotting, mass spectrometry, and the use of a p13 deletion mutant strain. The results suggested that P13 is the protein responsible for the 0.6-nanosiemens pore-forming activity in the outer membrane of B. burgdorferi.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Thein, Marcus
(författare)
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Barbot, Mariam
(författare)
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Sans-Serramitjana, Eulalia
(författare)
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Bonde, MariUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)maiboe01
(författare)
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Mentele, Reinhard
(författare)
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Lottspeich, Friedrich
(författare)
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Bergström, SvenUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)svbe0001
(författare)
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Benz, Roland
(författare)
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Umeå universitetInstitutionen för molekylärbiologi (Medicinska fakulteten)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Biological Chemistry: American Society for Biochemistry and Molecular Biology289:27, s. 18614-186240021-92581083-351X
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