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On-line desalting a...
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Bengtsson, JörgenUppsala universitet,Avdelningen för farmakokinetik och läkemedelsterapi,Hammarlund-Udenaes
(författare)
On-line desalting and determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide in microdialysis and plasma samples using column switching and liquid chromatography/tandem mass spectrometry
- Artikel/kapitelEngelska2005
Förlag, utgivningsår, omfång ...
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2005
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John Wiley & Sons,2005
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-103231
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-103231URI
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https://doi.org/10.1002/rcm.2035DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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A sensitive and reproducible method for the determination of morphine and the metabolites morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) was developed. The method was validated for perfusion fluid used in microdialysis as well as for sheep and human plasma. A C18 guard column was used to desalt the samples before analytical separation on a ZIC HILIC (hydrophilic interaction chromatography) column and detection with tandem mass spectrometry (MS/MS). The mobile phases were 0.05% trifluoroacetic acid (TFA) for desalting and acetonitrile/5 mM ammonium acetate (70:30) for separation. Microdialysis samples (5 microL) were directly injected onto the system. The lower limits of quantification (LLOQ) for morphine, M3G and M6G were 0.50, 0.22 and 0.55 ng/mL, respectively, and the method was linear from LLOQ to 200 ng/mL. For plasma, a volume of 100 microL was precipitated with acetonitrile containing internal standards (deuterated morphine and metabolites). The supernatant was evaporated and reconstituted in 0.05% TFA before the desalting process. The LLOQs for sheep plasma were 2.0 and 3.1 ng/mL and the ranges were 2.0-2000 and 3.1-3100 ng/mL for morphine and M3G, respectively. For human plasma, the LLOQs were 0.78, 1.49 and 0.53 ng/mL and the ranges were 0.78-500, 1.49-1000 and 0.53-500 ng/mL for morphine, M3G and M6G, respectively.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Jansson, BrittUppsala universitet,Avdelningen för farmakokinetik och läkemedelsterapi,Hammarlund-Udenaes(Swepub:uu)bja28141
(författare)
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Hammarlund-Udenaes, MargaretaUppsala universitet,Avdelningen för farmakokinetik och läkemedelsterapi,Hammarlund-Udenaes(Swepub:uu)marghamm
(författare)
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Uppsala universitetAvdelningen för farmakokinetik och läkemedelsterapi
(creator_code:org_t)
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Ingår i:Rapid Communications in Mass Spectrometry: John Wiley & Sons19:15, s. 2116-21220951-41981097-0231
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