Identification of Tyr900 in the kinase domain of c-Kit as a Src-dependent phosphorylation site mediating interaction with c-Crk

• We have previously demonstrated that ligand-stimulation of c-Kit induces phosphorylation of Tyr568 and Tyr570 in the juxtamembrane region of the receptor, leading to recruitment, phosphorylation and activation of members of the Src family of tyrosine kinases. In this paper, we demonstrate that members of the Src family of tyrosine kinases are able to phosphorylate c-Kit selectively on one particular tyrosine residue, Tyr900, located in the second part of the tyrosine kinase domain. In order to identify potential docking partners of Tyr900, a synthetic phosphopeptide corresponding to the amino acid sequence surrounding Tyr900 was used as an affinity matrix. By use of MALDI-TOF mass spectrometry, CrkII was identified as a protein that specifically bound to Tyr900 in a phosphorylation dependent manner, possibly via the p85 subunit of PI3-kinase. Expression of a mutant receptor where Tyr900 had been replaced with a phenylalanine residue (Y900F) resulted in a receptor with reduced ability to phosphorylate CrkII. Together these data support a model where c-Src phosphorylates the receptor, thereby creating docking sites for SH2 domain containing proteins, leading to recruitment of Crk to the receptor.

Nyckelord

3T3 Cells

Amino Acid Sequence

Animals

Aorta

Binding Sites

Endothelium; Vascular/cytology

Humans

Mice

Mutagenesis; Site-Directed

Phosphopeptides/metabolism

Phosphorylation

Protein Binding

Proto-Oncogene Proteins/metabolism

Proto-Oncogene Proteins c-crk

Proto-Oncogene Proteins c-kit/genetics/*metabolism

Swine

Transfection

Tyrosine/*metabolism

src Homology Domains

src-Family Kinases/*metabolism

Publikations- och innehållstyp

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