Sökning: onr:"swepub:oai:DiVA.org:uu-106189" >
Detection of Haemop...
Detection of Haemophilus influenzae in respiratory secretions from pneumonia patients by quantitative real-time polymerase chain reaction
-
- Abdeldaim, Guma M. K. (författare)
- Uppsala universitet,Klinisk bakteriologi,Björn Herrmann
-
- Strålin, Kristoffer (författare)
- Department of Infectious Diseases, Örebro University Hospital
-
- Kirsebom, Leif A. (författare)
- Uppsala universitet,Mikrobiologi,Leif Kirsebom
-
visa fler...
-
- Olcén, Per (författare)
- Department of Clinical Microbiology, Örebro University Hospital
-
- Blomberg, Jonas (författare)
- Uppsala universitet,Klinisk virologi,Jonas Blomberg
-
- Herrmann, Björn (författare)
- Uppsala universitet,Klinisk bakteriologi,Björn Herrmann
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2009
- 2009
- Engelska.
-
Ingår i: Diagnostic microbiology and infectious disease. - : Elsevier. - 0732-8893 .- 1879-0070. ; 64:4, s. 366-373
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A quantitative real-time polymerase chain reaction (PCR) based on the omp P6 gene was developed to detect Haemophilus influenzae. Its specificity was determined by analysis of 29 strains of 11 different Haemophilus spp. and was compared with PCR assays having other target genes: rnpB, 16S rRNA, and bexA. The method was evaluated on nasopharyngeal aspirates from 166 adult patients with community-acquired pneumonia. When 104 DNA copies/mL was used as cutoff limit for the method, P6 PCR had a sensitivity of 97.5% and a specificity of 96.0% compared with the culture. Of 20 culture-negative but P6 PCR-positive cases, 18 were confirmed by fucK PCR as H. influenzae. Five (5.9%) of 84 nasopharyngeal aspirates from adult controls tested PCR positive. We conclude that the P6 real-time PCR is both sensitive and specific for identification of H. influenzae in respiratory secretions. Quantification facilitates discrimination between disease-causing H. influenzae strains and commensal colonization.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- H. influenzae
- Pneumonia
- Real-time PCR
- Outer membrane protein P6
- fucK
- rnpB
- Clinical bacteriology
- Klinisk bakteriologi
- Mikrobiologi
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas