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Type I membrane Klo...
Type I membrane Klotho expression is decreased and inversely correlated to serum calcium in primary hyperparathyroidism
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- Björklund, Peyman (författare)
- Uppsala universitet,Endokrinkirurgi
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- Krajisnik, Tijana, 1981- (författare)
- Uppsala universitet,Medicin,Metabola bensjukdomar, fosfatmetabolism
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- Åkerström, Göran (författare)
- Uppsala universitet,Endokrinkirurgi
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- Westin, Gunnar (författare)
- Uppsala universitet,Endokrinkirurgi
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- Larsson, Tobias E. (författare)
- Uppsala universitet,Medicin,Metabola bensjukdomar, fosfatmetabolism
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(creator_code:org_t)
- The Endocrine Society, 2008
- 2008
- Engelska.
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 0021-972X .- 1945-7197. ; 93:10, s. 4152-4157
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Context: The type I membrane protein Klotho was recently shownto mediate PTH secretion in parathyroid cells in response tolow extracellular calcium. In contrast, Klotho inhibits PTHsecretion indirectly through the action of fibroblast growthfactor-23. Abnormal Klotho expression in parathyroid disordersremains to be elucidated.Objective: The aim of the study was to determine: 1) Klothoexpression in parathyroid adenomas from patients with primaryhyperparathyroidism (pHPT) compared to normal tissue; and 2)its relation to the serum calcium and PTH levels.Design: Surgically removed parathyroid glands (n = 40) and fournormal parathyroid tissue specimens were analyzed for KlothomRNA and protein levels by quantitative real-time PCR and immunohistochemistry.In vitro effects of calcium on Klotho mRNA expression were studiedin bovine parathyroid cells.Results: Klotho mRNA levels were significantly decreased (n= 23) or undetectable (n = 17) in parathyroid adenomas comparedto normal tissues (P < 0.001). Reduced Klotho protein expressionwas confirmed by immunohistochemistry. Klotho mRNA levels wereinversely correlated to serum calcium (r = –0.97; P <0.0001), and calcium dose-dependently decreased Klotho mRNAexpression in normal parathyroid cells in vitro (P < 0.01).Serum calcium was the only significant marker of Klotho expressionin multivariate analysis with calcium, phosphate, PTH, and adenomaweight as independent variables.Conclusions: Parathyroid Klotho expression is decreased or undetectablein pHPT. We provide evidence that 1) serum calcium is stronglyassociated with parathyroid Klotho expression in pHPT; and 2)abnormal PTH secretion in hypercalcemic pHPT subjects is mediatedby Klotho-independent mechanisms.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Klotho
- Fibroblast growth factor-23
- FGF23
- FGF-23
- pHPT
- adenoma
- primary hyperparathyroidism
- calcium
- Endocrinology
- Endokrinologi
- Medicin
- Medicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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