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Electron transfer p...
Electron transfer protein complexes in the thylakoid membranes of heterocysts from the cyanobacterium Nostoc punctiforme
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- Cardona, Tanai, 1983- (författare)
- Uppsala universitet,Molekylär biomimetik
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- Battchikova, Natalia (författare)
- Department of Biology, University of Turku,Laboratory of Plant Physiology and Molecular Biology
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- Zhang, Pengpeng (författare)
- Department of Biology, University of Turku,Laboratory of Plant Physiology and Molecular Biology
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- Stensjö, Karin (författare)
- Uppsala universitet,Mikrobiell kemi
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- Aro, Eva-Mari (författare)
- Department of Biology, University of Turku,Laboratory of Plant Physiology and Molecular Biology
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- Lindblad, Peter (författare)
- Uppsala universitet,Mikrobiell kemi
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- Magnuson, Ann (författare)
- Uppsala universitet,Molekylär biomimetik
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(creator_code:org_t)
- Elsevier, 2009
- 2009
- Engelska.
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Ingår i: Biochimica et Biophysica Acta - Bioenergetics. - : Elsevier. - 0005-2728 .- 1879-2650. ; 1787:4, s. 252-263
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Filamentous, heterocystous cyanobacteria are capable of nitrogen fixation and photoautotrophic growth. Nitrogen fixation takes place in heterocysts that differentiate as a result of nitrogen starvation. Heterocysts uphold a microoxic environment to avoid inactivation of nitrogenase, e.g. by downregulation of oxygenic photosynthesis. The ATP and reductant requirement for the nitrogenase reaction is considered to depend on Photosystem I, but little is known about the organization of energy converting membrane proteins in heterocysts. We have investigated the membrane proteome of heterocysts from nitrogen fixing filaments of Nostoc punctiforme sp. PCC 73102, by 2D gel electrophoresis and mass spectrometry. The membrane proteome was found to be dominated by the Photosystem I and ATP-synthase complexes.We could identify asignificant amount of assembled Photosystem II complexes containing the D1, D2, CP43, CP47 and PsbO proteins from these complexes. We could also measure light-driven in vitro electron transfer from Photosystem II in heterocyst thylakoid membranes. We did not find any partially disassembled PhotosystemII complexes lacking the CP43 protein. Several subunits of the NDH-1 complex were also identified. The relative amount of NDH-1M complexes was found to be higher than NDH-1L complexes, which might suggest a role for this complex in cyclic electron transfer in the heterocysts of Nostoc punctiforme.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Cyanobacteria
- Nostoc
- Heterocyst
- Photosystem II
- Fluorescence
- BN-PAGE
- Chemistry
- Kemi
- Biokemi
- Biochemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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