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Calliandra selloi M...
Calliandra selloi Macbride trypsin inhibitor : isolation, characterization, stability, spectroscopic analyses
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Yoshizaki, Lucila (författare)
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Troncoso, María F. (författare)
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Lopes, Jose L. S. (författare)
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- Hellman, Ulf (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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Beltramini, Leila M. (författare)
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Wolfenstein-Todel, Carlota (författare)
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 68:21, s. 2625-2634
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A trypsin inhibitor was purified from Calliandra selloi Macbride seeds (CSTI). SDS-PAGE under non-reducing conditions showed a single band of approximately 20,000Da, while under reducing conditions two bands of 16,000 and 6000Da were observed, indicating that CSTI consists of two polypeptide chains. Molecular masses of 20,078 and 20,279 were obtained by mass spectrometry, although only one pI of 4.0 was observed and one peak was obtained by reversed phase chromatography. Amino-terminal sequence analysis showed homology to Kunitz-type inhibitors. CSTI was able to inhibit trypsin (K(i) 2.21x10(-7)M), alpha-chymotrypsin (K(i) 4.95x10(-7)M) and kallikrein (K(i) 4.20x10(-7)M) but had no effect on elastase. Trypsin inhibitory activity was stable over a wide range of pH and temperature. CSTI was particularly susceptible to DTT treatment, followed by addition of iodoacetamide. Far-UV circular dichroism measurements revealed that CSTI is a beta-II protein. Thermal unfolding showed a two-state transition with a midpoint at 68 degrees C. Far-UV CD spectra of CSTI at pH extremes showed little changes, while more pronounced differences in near-UV CD spectra were detected. Remarkably, treatment with 1mM DTT caused very slight changes in the far-UV CD spectrum, and only after carbamidomethylation was there was a marked loss observed in secondary structure.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Calliandra selloi MacBride
- Characterization
- Kunitz-type trypsin inhibitor
- Leguminosae
- Protein purification
- Stability studies
- MEDICINE
- MEDICIN
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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