Sökning: onr:"swepub:oai:DiVA.org:uu-120036" > Dual Targeting to M...
Fältnamn | Indikatorer | Metadata |
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000 | 04920naa a2200553 4500 | |
001 | oai:DiVA.org:uu-120036 | |
003 | SwePub | |
008 | 100305s2009 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:su-31102 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1200362 URI |
024 | 7 | a https://doi.org/10.1093/mp/ssp0482 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-311022 URI |
040 | a (SwePub)uud (SwePub)su | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Berglund, Anna-Karinu Stockholms universitet,Institutionen för biokemi och biofysik,Elzbieta Glaser4 aut0 (Swepub:su)annab |
245 | 1 0 | a Dual Targeting to Mitochondria and Chloroplasts :b Characterization of Thr–tRNA Synthetase Targeting Peptide |
264 | 1 | a Shanghai :b Oxford University Press,c 2009 |
338 | a print2 rdacarrier | |
500 | a 462IGP | |
520 | a There is a group of proteins that are encoded by a single gene, expressed as a single precursor protein and dually targeted to both mitochondria and chloroplasts using an ambiguous targeting peptide. Sequence analysis of 43 dual targeted proteins in comparison with 385 mitochondrial proteins and 567 chloroplast proteins of Arabidopsis thaliana revealed an overall significant increase in phenylalanines, leucines, and serines and a decrease in acidic amino acids and glycine in dual targeting peptides (dTPs). The N-terminal portion of dTPs has significantly more serines than mTPs. The number of arginines is similar to those in mTPs, but almost twice as high as those in cTPs. We have investigated targeting determinants of the dual targeting peptide of Thr-tRNA synthetase (ThrRS-dTP) studying organellar import of N- and C-terminal deletion constructs of ThrRS-dTP coupled to GFP. These results show that the 23 amino acid long N-terminal portion of ThrRS-dTP is crucial but not sufficient for the organellar import. The C-terminal deletions revealed that the shortest peptide that was capable of conferring dual targeting was 60 amino acids long. We have purified the ThrRS-dTP(2-60) to homogeneity after its expression as a fusion construct with GST followed by CNBr cleavage and ion exchange chromatography. The purified ThrRS-dTP(2-60) inhibited import of pF(1)beta into mitochondria and of pSSU into chloroplasts at mu M concentrations showing that dual and organelle-specific proteins use the same organellar import pathways. Furthermore, the CD spectra of ThrRS-dTP(2-60) indicated that the peptide has the propensity for forming alpha-helical structure in membrane mimetic environments; however, the membrane charge was not important for the amount of induced helical structure. This is the first study in which a dual targeting peptide has been purified and investigated by biochemical and biophysical means. | |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a Dual targeting | |
653 | a mitochondria | |
653 | a chloroplast | |
653 | a targeting peptide | |
653 | a aminoacyl–tRNA synthetase | |
653 | a Biology | |
653 | a Biologi | |
653 | a Biology with specialization in Molecular Biology | |
653 | a Biologi med inriktning mot molekylärbiologi | |
700 | 1 | a Spånning, Erikau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)ersp4638 |
700 | 1 | a Biverståhl, Henriku Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)hbive |
700 | 1 | a Maddalo, Gianlucau Stockholms universitet,Institutionen för analytisk kemi4 aut0 (Swepub:su)gmadd |
700 | 1 | a Tellgren-Roth, Christian,d 1971-u Uppsala universitet,Genomik,Ulf Gyllensten4 aut0 (Swepub:uu)chrte903 |
700 | 1 | a Mäler, Lenau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)maler |
700 | 1 | a Glaser, Elzbietau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)glase |
710 | 2 | a Stockholms universitetb Institutionen för biokemi och biofysik4 org |
773 | 0 | t Molecular Plantd Shanghai : Oxford University Pressg 2:6, s. 1298-1309q 2:6<1298-1309x 1674-2052 |
856 | 4 | u http://mplant.oxfordjournals.org/cgi/content/full/2/6/1298 |
856 | 4 | u http://www.cell.com/article/S1674205214605062/pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-120036 |
856 | 4 8 | u https://doi.org/10.1093/mp/ssp048 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-31102 |
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