Sökning: onr:"swepub:oai:DiVA.org:uu-129430" > Biotinylated lipid ...
Fältnamn | Indikatorer | Metadata |
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000 | 03391naa a2200457 4500 | |
001 | oai:DiVA.org:uu-129430 | |
003 | SwePub | |
008 | 100815s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1294302 URI |
024 | 7 | a https://doi.org/10.1016/j.ab.2010.06.0302 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Lundquist, Annau Uppsala universitet,Institutionen för fysikalisk och analytisk kemi4 aut0 (Swepub:uu)anlun534 |
245 | 1 0 | a Biotinylated lipid bilayer disks as model membranes for biosensor analyses |
264 | 1 | b Elsevier BV,c 2010 |
338 | a print2 rdacarrier | |
520 | a The aim of this study was to investigate the potential of polyethylene glycol (PEG)-stabilized lipid bilayer disks as model membranes for surface plasmon resonance (SPR)-based biosensor analyses. Nanosized bilayer disks that included 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)(2000)] (DSPE-PEG(2000)-biotin) were prepared and structurally characterized by cryo-transmission electron microscopy (cryo-TEM) imaging. The biotinylated disks were immobilized via streptavidin to three different types of sensor chips (CM3, CM4, and CM5) varying in their degree of carboxymethylation and thickness of the dextran matrix. The bilayer disks were found to interact with and bind stably to the streptavidin-coated sensor surfaces. As a first step toward the use of these bilayer disks as model membranes in SPR-based studies of membrane proteins, initial investigations were carried out with cyclooxygenases 1 and 2 (COX 1 and COX 2). Bilayer disks were preincubated with the respective protein and thereafter allowed to interact with the sensor surface. The signal resulting from the interaction was, in both cases, significantly enhanced as compared with the signal obtained when disks alone were injected over the surface. The results of the study suggest that bilayer disks constitute a new and promising type of model membranes for SPR-based biosensor studies. | |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
653 | a Lipid bilayer disk | |
653 | a Liposome | |
653 | a Immobilization | |
653 | a Streptavidin | |
653 | a Biotin | |
653 | a Biacore | |
653 | a Surface plasmon resonance | |
653 | a SPR | |
653 | a Biosensor | |
653 | a Chemistry | |
653 | a Kemi | |
700 | 1 | a Hansen, Søren Bu Uppsala universitet,Institutionen för fysikalisk och analytisk kemi4 aut |
700 | 1 | a Nordström, Helenau Uppsala universitet,Institutionen för biokemi och organisk kemi4 aut0 (Swepub:uu)henor020 |
700 | 1 | a Danielson, U Helenau Uppsala universitet,Institutionen för biokemi och organisk kemi4 aut0 (Swepub:uu)helenads |
700 | 1 | a Edwards, Katarinau Uppsala universitet,Institutionen för fysikalisk och analytisk kemi4 aut0 (Swepub:uu)kataedwa |
710 | 2 | a Uppsala universitetb Institutionen för fysikalisk och analytisk kemi4 org |
773 | 0 | t Analytical Biochemistryd : Elsevier BVg 405:2, s. 153-159q 405:2<153-159x 0003-2697x 1096-0309 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-129430 |
856 | 4 8 | u https://doi.org/10.1016/j.ab.2010.06.030 |
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