Search: id:"swepub:oai:DiVA.org:uu-13000" >
Upregulation of bfl...
Upregulation of bfl-1 is a potential mechanism of chemoresistance in B-cell chronic lymphocytic leukaemia
-
- Olsson, A. (author)
- Karolinska Institutet
-
- Norberg, Maria (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
Ökvist, A. (author)
-
show more...
-
Derkow, K. (author)
-
- Choudhury, A. (author)
- Karolinska Institutet
-
- Tobin, Gerard (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
Celsing, F. (author)
-
- Österborg, A. (author)
- Karolinska Institutet
-
- Rosenquist, Richard (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Jondal, M. (author)
- Karolinska Institutet
-
- Osorio, L. M. (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2007-08-28
- 2007
- English.
-
In: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 97:6, s. 769-777
- Related links:
-
https://www.nature.c...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- B-cell chronic lymphocytic leukaemia (B-CLL) is characterised by the progressive accumulation of monoclonal CD5+ B cells. In a previous study, we have analysed the expression profile of apoptosis-regulating genes using a cDNA-based microarray and found overexpression of the antiapoptotic bcl-2 family member, bfl-1, in B-CLL cells with an apoptosis-resistant phenotype. In this study, bfl-1 mRNA levels have been determined by competitive PCR in an extended population of B-CLL patients to characterise its role in disease progression and development of chemoresistance. bfl-1 levels were significantly higher in patients with no response (NR) to last chemotherapy than in patients responding (partial response (PR)) to last chemotherapy (P<0.05) and in patients who had not required treatment (P<0.05). We found no correlation between bfl-1 mRNA levels and disease progression, IGHV mutational status or other clinical parameters. In addition, bfl-1 mRNA levels were inversely correlated with apoptotic response to in vitro fludarabine treatment of B-CLL cells. Specific downregulation of bfl-1 using siRNA induced apoptosis in resistant cells. Our data suggest that bfl-1 contributes to chemoresistance and might be a therapeutic target in B-CLL.
Keyword
- Apoptosis
- B-CLL
- Bfl-1
- Chemoresistance
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Olsson, A.
-
Norberg, Maria
-
Ökvist, A.
-
Derkow, K.
-
Choudhury, A.
-
Tobin, Gerard
-
show more...
-
Celsing, F.
-
Österborg, A.
-
Rosenquist, Rich ...
-
Jondal, M.
-
Osorio, L. M.
-
show less...
- Articles in the publication
-
British Journal ...
- By the university
-
Uppsala University
-
Karolinska Institutet