Sökning: onr:"swepub:oai:DiVA.org:uu-130667" > Quantification of i...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03772naa a2200481 4500 | |
001 | oai:DiVA.org:uu-130667 | |
003 | SwePub | |
008 | 100910s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1306672 URI |
024 | 7 | a https://doi.org/10.3892/ijo_000005512 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Göstring, Lovisau Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut |
245 | 1 0 | a Quantification of internalization of EGFR-binding Affibody molecules :b Methodological aspects |
264 | 1 | b Spandidos Publications,c 2010 |
338 | a print2 rdacarrier | |
520 | a Tumor cell internalization of targeting agents is of interest, since internalization influences the local retention time of a radionuclide and thereby imaging quality in PET and SPECT and effects of radionuclide therapy. In cases where nuclear methods are not applicable at the cellular level, quantitative fluorescent techniques are useful as described in this article. Two fluorescence-based methods to study cellular internalization were applied: the CypHer and the Alexa488-quenching methods, both utilized in fluorescence microscopy and flow cytometry. Two EGFR-binding Affibody molecules were analyzed in A431 cells: the monomer Z1907 and the dimer (Z1907)2. EGF, cetuximab and non-specific Affibody molecules were used as controls. For comparison, internalization of 111In-labeled Z1907 was studied with the acid wash internalization assay. The Cypher method is straightforward, but requires equal labeling of all compounds for accurate quantification. The Alexa488-quenching method is preferable since it is independent of the dye-to-protein ratio. According to this method, about 45% of EGF and 19-24% of the bound Affibody molecules and cetuximab were internalized within one hour. Similar results were seen with 111In-Z1907 in the acid wash method, while (Z1907)2 was not removed by acid and thus could not be studied this way. The fluorescence-based Alexa488-quenching method is well suited to quantitatively analyze internalization of targeting agents, also those that resist acid wash. The internalized fraction showed that both the monomeric and dimeric Affibody molecules are expected to give good uptake and thereby good retention of metallic radionuclides which will render good tumor to background values. | |
653 | a 111In | |
653 | a A431 | |
653 | a Affibody molecule | |
653 | a Alexa488 | |
653 | a Cetuximab | |
653 | a CypHer5E | |
653 | a EGFR | |
653 | a Internalization | |
653 | a Radionuclide | |
653 | a Retention | |
653 | a MEDICINE | |
653 | a MEDICIN | |
700 | 1 | a Chew, Ming Tsueyu Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut |
700 | 1 | a Orlova, Annau Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut0 (Swepub:uu)annaorlo |
700 | 1 | a Höidén-Guthenberg, Ingmarie4 aut |
700 | 1 | a Wennborg, Anders4 aut |
700 | 1 | a Carlsson, Jörgenu Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut0 (Swepub:uu)jorgcarl |
700 | 1 | a Frejd, Fredrik Y.u Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut0 (Swepub:uu)freni195 |
710 | 2 | a Uppsala universitetb Enheten för biomedicinsk strålningsvetenskap4 org |
773 | 0 | t International Journal of Oncologyd : Spandidos Publicationsg 36:4, s. 757-763q 36:4<757-763x 1019-6439x 1791-2423 |
856 | 4 | u https://www.spandidos-publications.com/ijo/36/4/757/download |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130667 |
856 | 4 8 | u https://doi.org/10.3892/ijo_00000551 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.