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H2O2-induced Ca2+ i...
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Bari, Muhammad R.
(författare)
H2O2-induced Ca2+ influx and its inhibition by N-(p-amylcinnamoyl) anthranilic acid in the beta-cells : involvement of TRPM2 channels
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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2009-03-06
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Wiley,2009
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-132137
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132137URI
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https://doi.org/10.1111/j.1582-4934.2009.00737.xDOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:119993110URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Type 2 melastatin-related transient receptor potential channel (TRPM2), a member of the melastatin-related TRP (transient receptor potential) subfamily is a Ca(2+)-permeable channel activated by hydrogen peroxide (H(2)O(2)). We have investigated the role of TRPM2 channels in mediating the H(2)O(2)-induced increase in the cytoplasmic free Ca(2+) concentration ([Ca(2+)](i)) in insulin-secreting cells. In fura-2 loaded INS-1E cells, a widely used model of beta-cells, and in human beta-cells, H(2)O(2) increased [Ca(2+)](i), in the presence of 3 mM glucose, by inducing Ca(2+) influx across the plasma membrane. H(2)O(2)-induced Ca(2+) influx was not blocked by nimodipine, a blocker of the L-type voltage-gated Ca(2+) channels nor by 2-aminoethoxydiphenyl borate, a blocker of several TRP channels and store-operated channels, but it was completely blocked by N-(p-amylcinnamoyl)anthranilic acid (ACA), a potent inhibitor of TRPM2. Adenosine diphosphate phosphate ribose, a specific activator of TRPM2 channel and H(2)O(2), induced inward cation currents that were blocked by ACA. Western blot using antibodies directed to the epitopes on the N-terminal and on the C-terminal parts of TRPM2 identified the full length TRPM2 (TRPM2-L), and the C-terminally truncated TRPM2 (TRPM2-S) in human islets. We conclude that functional TRPM2 channels mediate H(2)O(2)-induced Ca(2+) entry in beta-cells, a process potently inhibited by ACA.
Ämnesord och genrebeteckningar
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Calcium influx
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Calcium signalling
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Insulin-secreting cells
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Microfluorometry
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N-(p-amylcinnamoyl)anthranilic acid
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TRP channels
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TRPM2
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Akbar, Sanian
(författare)
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Eweida, Mohamed
(författare)
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Kühn, Frank J. P.
(författare)
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Gustafsson, Amanda Jabin
(författare)
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Lückhoff, Andreas
(författare)
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Islam, M. ShahidulKarolinska Institutet,Uppsala universitet,Medicin
(författare)
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Uppsala universitetMedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Cellular and Molecular Medicine: Wiley13:9B, s. 3260-32671582-18381582-4934
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