The Src homology 2 domain-containing adapter protein B (SHB) regulates mouse oocyte maturation

Calounova, GabrielaUppsala universitet,Institutionen för medicinsk cellbiologi (författare)

SHB (Src homology 2 domain-containing adapter protein B) is involved in receptor tyrosine kinase signaling. Mice deficient in the Shb gene have been found to exhibit a transmission ratio distortion with respect to inheritance of the Shb null allele among offspring and this phenomenon was linked to female gamete production. Consequently, we postulated that Shb plays a role for oocyte biology and thus decided to investigate oocyte formation, meiotic maturation, and early embryo development in relation to absence of the Shb gene. Oogenesis was apparently accelerated judging from the stages of oocyte development on fetal day 18.5 and one week postnatally in Shb -/- mice; but in adulthood ovarian follicle maturation was impaired in these mice. Completion of meiosis I (first polar body extrusion) was less synchronized, with a fraction of oocytes showing premature polar body extrusion in the absence of Shb. In vitro fertilization of mature oocytes isolated from Shb +/+, +/- and -/- mice revealed impaired early embryo development in the -/- embryos. Moreover, the absence of Shb enhanced ERK (extracellular-signal regulated kinase) and RSK (ribosomal S6 kinase) signaling in oocytes and these effects were paralleled by an increased ribosomal protein S6 phosphorylation and activation. It is concluded that SHB regulates normal oocyte and follicle development and that perturbation of SHB signaling causes defective meiosis I and early embryo development.

Livera, GabrielINSERM/CEA/paris Diderot-Paris 7 (författare)
Zhang, X-QUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)xiaozhan (författare)
Liu, KuiUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umeå Universitet (författare)
Gosden, Roger G (författare)
Welsh, MichaelUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)michwels (författare)
Uppsala universitetInstitutionen för medicinsk cellbiologi (creator_code:org_t)

Av författaren/redakt...: Calounova, Gabri ...; Livera, Gabriel; Zhang, X-Q; Liu, Kui; Gosden, Roger G; Welsh, Michael

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