Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-Friendly Solvents

Co, Michelle,1975-Uppsala universitet,Analytisk kemi,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala (författare)

Introduction-Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants.Objective- To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark.Methodology- A screening experiment that involved three different techniques, was conducted to determine the best technique to extract antioxidants.The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-2’-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants.Results- PFE using water and ethanol as solvent at 160 and 180°C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts.Conclusion-The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Andra medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Other Basic Medicine hsv//eng
NATURVETENSKAP Kemi Organisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Organic Chemistry hsv//eng
Accelerated solvent extraction
antioxidant
DPPH
ethanol
Picea abies
pressurised fluid extraction
supercritical fluid extraction
water
Chemistry
Kemi
Analytical Chemistry
Analytisk kemi
accelerated solvent extraction
antioxidant
DPPH
ethanol
Picea abies
pressurised fluid extraction
supercritical fluid extraction
water
stilbene glucosides

Fagerlund, AmelieMittuniversitetet,Uppsala universitet,Institutionen för biokemi och organisk kemi,Institutionen för tillämpad naturvetenskap och design (-2013)(Swepub:miun)amefag (författare)
Engman, LarsUppsala universitet,Institutionen för kemi - BMC,Dept Biochem & Organ Chem, Uppsala Univ, SE-75123 Uppsala(Swepub:uu)len21181 (författare)
Sunnerheim, KerstinMittuniversitetet,Institutionen för tillämpad naturvetenskap och design (-2013),Department of Natural Sciences, Engineering and Mathematics, Mid Sweden University(Swepub:miun)kersun (författare)
Sjöberg, Per J. RUppsala universitet,Analytisk kemi,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala(Swepub:uu)psj18455 (författare)
Turner, CharlottaLund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala,Dept of Organic Chemistry, Lund University(Swepub:lu)orga-cht (författare)
Uppsala universitetAnalytisk kemi (creator_code:org_t)

Av författaren/redakt...: Co, Michelle, 19 ...; Fagerlund, Ameli ...; Engman, Lars; Sunnerheim, Kers ...; Sjöberg, Per J. ...; Turner, Charlott ...

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