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The CyAbrB transcri...
The CyAbrB transcription factor CalA regulates the iron superoxide dismutase in Nostoc sp. strain PCC 7120
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- Agervald, Åsa (författare)
- Uppsala universitet,Mikrobiell kemi
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- Baebprasert, Wipawee (författare)
- Uppsala universitet,Mikrobiell kemi
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- Zhang, Xiaohui (författare)
- Uppsala universitet,Mikrobiell kemi
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Incharoensakdi, Aran (författare)
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- Lindblad, Peter (författare)
- Uppsala universitet,Mikrobiell kemi
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- Stensjö, Karin (författare)
- Uppsala universitet,Mikrobiell kemi
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(creator_code:org_t)
- Wiley, 2010
- 2010
- Engelska.
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Ingår i: Environmental Microbiology. - : Wiley. - 1462-2912 .- 1462-2920. ; 12:10, s. 2826-2837
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- P>In the present investigation the results of induced over-production of the CyAbrB transcription factor CalA (Cyanobacterial AbrB-like, annotated as Alr0946) in the cyanobacterium Nostoc sp. PCC 7120 were analysed. The CalA overexpression strain showed a bleaching phenotype with lower growth rate and truncated filaments 2 days after induction of overexpression. The phenotype was even more pronounced when illumination was increased from 35 to 125 mu mol m-2 s-1. Using gel-based quantitative proteomics, the induced overexpression of CalA was shown to downregulate the abundance of FeSOD, one of two types of superoxide dismutases in Nostoc sp. PCC 7120. The change in protein abundance was also accompanied by lower transcript as well as activity levels. Purified recombinant CalA from Nostoc sp. PCC 7120 was shown to interact with the promoter region of alr2938, encoding FeSOD, indicating a transcriptional regulation of FeSOD by CalA. The bleaching phenotype is in line with a decreased tolerance against oxidative stress and indicates that CalA is involved in regulation of cellular responses in which FeSOD has an important and specific function in the filamentous cyanobacterium Nostoc sp. PCC 7120.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Biology
- Biologi
- Chemistry
- Kemi
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- ref (ämneskategori)
- art (ämneskategori)
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