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Sökning: onr:"swepub:oai:DiVA.org:uu-136069" > MS analysis of rheu...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003783naa a2200505 4500
001oai:DiVA.org:uu-136069
003SwePub
008101209s2010 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:120565229
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1360692 URI
0247 a https://doi.org/10.1002/prca.2009000882 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1205652292 URI
040 a (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Hermansson, Monika4 aut
2451 0a MS analysis of rheumatoid arthritic synovial tissue identifies specific citrullination sites on fibrinogen.
264 c 2010-01-25
264 1b Wiley,c 2010
338 a print2 rdacarrier
520 a Purpose Citrullination is a post-translational modification of arginine residues to citrulline catalyzed by peptidyl arginine deiminases. Induced expression of citrullinated proteins are frequently detected in various inflammatory states including arthritis; however, direct detection of citrullination in arthritic samples has not been successfully performed in the past. Experimental design Citrullination of human fibrinogen, a candidate autoantigen in arthritis, was studied. Accurate identification of citrullinated fibrinogen peptides from rheumatoid arthritis synovial tissue specimens was performed using accurate mass and retention time analysis. Results A peptide with the sequence ESSSHHPGIAEFPSRGK corresponding to amino acids 559-575 of fibrinogen a-chain was identified to be citrullinated with an occupancy rate between 1.4 and 2.5%. Citrullination of the peptide KREEAPSLRPAPPPISGGGYRARPAK corresponding to amino acids 52-77 of the fibrinogen beta-chain was identified with an occupancy rate of 1.2%. Conclusions and clinical relevance We report a proof of principle study for the identification of citrullinated proteins and within them, identification of citrullination sites and quantification of their occupancies in synovial tissue from rheumatoid arthritis patients using high-resolution MS. Detailed studies on which molecules are citrullinated in arthritis can provide information about their role in immune regulation and serve as novel biomarkers and potentially even as therapeutic targets.
653 a Citrullination
653 a fibrinogen
653 a MS
653 a rheumatoid arthritis
653 a MEDICINE
653 a MEDICIN
7001 a Artemenko, Konstantinu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)konar476
7001 a Ossipova, Elenau Karolinska Institutet4 aut
7001 a Eriksson, Hannau Karolinska Institutet4 aut
7001 a Lengqvist, Johanu Karolinska Institutet4 aut
7001 a Makrygiannakis, Dimitrios4 aut
7001 a Catrina, Anca I.u Karolinska Institutet4 aut
7001 a Nicholas, Anthony P.4 aut
7001 a Klareskog, Larsu Karolinska Institutet4 aut
7001 a Savitski, Mikhailu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut
7001 a Zubarev, Roman A.u Karolinska Institutet,Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)rozub839
7001 a Jakobsson, Per-Johanu Karolinska Institutet4 aut
7102 a Uppsala universitetb Institutionen för cell- och molekylärbiologi4 org
7730 t Proteomics - Clinical Applicationsd : Wileyg 4:5, s. 511-518q 4:5<511-518x 1862-8354x 1862-8346
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-136069
8564 8u https://doi.org/10.1002/prca.200900088
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:120565229

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