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Beta-globin mRNA in...
Beta-globin mRNA increases rapidly during erythropoietin treatment
- Artikel/kapitelEngelska2003
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LIBRIS-ID:oai:DiVA.org:uu-137253
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-137253URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Recombinant human erythropoietin (r-HuEpo) has an important role in the treatment of anaemic patients. Because of the high cost of r-HuEpo treatment, an early indicator of whether a patient is responding to the therapy would be valuable. Although measurement of gene expression is a promising new tool, it has not yet been established in clinical practice. The response pattern of a possible new marker, beta-globin mRNA, is compared with reticulocyte count, levels of haemoglobin, transferrin receptor and ferritin after r-HuEpo treatment. Eight healthy volunteers were stimulated with erythropoietin three times a week for four weeks and compared with five untreated control subjects. Blood samples were collected before each erythropoietin injection. Quantitative measurement of beta-globin mRNA was performed by poly(A) selection onto a manifold plastic support, coated with oligo(dT). The mRNA was reverse transcribed, followed by quantitative analysis using PCR via the 5' nuclease assay. The individuals treated with rHuEpo showed a more distinct increase in beta-globin mRNA levels than all other laboratory measurements. Beta-globin mRNA levels are therefore promising as a marker for the response to treatment with Epo.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Barbany, G.
(författare)
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Landegren, U.
(författare)
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Birgegård, Gunnar,1944-Uppsala universitet,Institutionen för medicinska vetenskaper,Hematologi
(författare)
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Uppsala universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scandinavian Journal of Clinical and Laboratory Investigation63:3, s. 239-2450036-55131502-7686
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