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Coupling oligo-β-cy...
Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin
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Yang, Li (författare)
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Zhu, Yan (författare)
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Tan, Tianwei (författare)
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- Janson, Jan-Christer (författare)
- Uppsala universitet,Ytbioteknik
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Process Biochemistry. - : Elsevier BV. - 1359-5113 .- 1873-3298. ; 42:7, s. 1075-1083
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The isoflavonoid puerarin, a well-known bioactive constituent in traditional Chinese medicine, can be separated from other isoflavonoids in the extracts of Radix puerariae (root of the plant Pueraria lobata) on oligo-β-cyclodextrin ligand coupled to D152 beads. The parameters of coupling time, temperature, solvent system and molar ratio of reactants were tested to optimize the preparation conditions. Acetic acid (7%) was used as the optimum mobile phase. The fine particle sizes of the coupled microspheres packing displayed high resolving power and efficiency. Compared with other two polyacrylate carrier media, PGMA-CDP and PHEMA-CDP, various retention mechanisms such as β-CD inclusion complexation, hydrogen bond interaction, and π–π interaction might exist in the chromatography process. This study provides a novel alternative matrix for the separation of puerarin.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- β-Cyclodextrin
- Chromatography
- Polyacrylate beads media
- Coupling
- Separation
- Puerarin
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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