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Sökning: onr:"swepub:oai:DiVA.org:uu-148961" > A HupS-GFP fusion p...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03346naa a2200457 4500 | |
| 001 | oai:DiVA.org:uu-148961 | |
| 003 | SwePub | |
| 008 | 110314s2011 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1489612 URI |
| 024 | 7 | a https://doi.org/10.1111/j.1574-6968.2010.02206.x2 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Camsund, Danielu Uppsala universitet,Molekylär biomimetik,Mikrobiell kemi4 aut0 (Swepub:uu)danka387 |
| 245 | 1 0 | a A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme |
| 264 | c 2011-01-24 | |
| 264 | 1 | b John Wiley & Sons,c 2011 |
| 338 | a print2 rdacarrier | |
| 520 | a All diazotrophic filamentous cyanobacteria contain an uptake hydrogenase that is involved in the reoxidation of H-2 produced during N-2-fixation. In Nostoc punctiforme ATCC 29133, N-2-fixation takes place in the microaerobic heterocysts, catalysed by a nitrogenase. Although the function of the uptake hydrogenase may be closely connected to that of nitrogenase, the localization in cyanobacteria has been under debate. Moreover, the subcellular localization is not understood. To investigate the cellular and subcellular localization of the uptake hydrogenase in N. punctiforme, a reporter construct consisting of the green fluorescent protein (GFP) translationally fused to HupS, within the complete hupSL operon, was constructed and transferred into N. punctiforme on a self-replicative vector by electroporation. Expression of the complete HupS-GFP fusion protein was confirmed by Western blotting using GFP antibodies. The N. punctiforme culture expressing HupS-GFP was examined using laser scanning confocal microscopy, and fluorescence was exclusively detected in the heterocysts. Furthermore, the fluorescence in mature heterocysts was localized to several small or fewer large clusters, which indicates a specificity of the subcellular localization of the uptake hydrogenase. | |
| 653 | a HupS | |
| 653 | a GFP | |
| 653 | a cyanobacteria | |
| 653 | a fusion protein | |
| 653 | a overlap-extension PCR | |
| 653 | a Hup | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 653 | a Medicin | |
| 653 | a Medicine | |
| 700 | 1 | a Devine, Ellenoru Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap4 aut |
| 700 | 1 | a Holmqvist, Marieu Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap4 aut0 (Swepub:uu)mahol120 |
| 700 | 1 | a Yohanoun, Peteru Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap4 aut |
| 700 | 1 | a Lindblad, Peteru Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap4 aut0 (Swepub:uu)peterlb |
| 700 | 1 | a Stensjö, Karinu Uppsala universitet,Mikrobiell kemi4 aut0 (Swepub:uu)kafor020 |
| 710 | 2 | a Uppsala universitetb Molekylär biomimetik4 org |
| 773 | 0 | t FEMS Microbiology Lettersd : John Wiley & Sonsg 316:2, s. 152-159q 316:2<152-159x 0378-1097x 1574-6968 |
| 856 | 4 | u https://academic.oup.com/femsle/article-pdf/316/2/152/19119948/316-2-152.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-148961 |
| 856 | 4 8 | u https://doi.org/10.1111/j.1574-6968.2010.02206.x |
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- Camsund, Daniel
- Devine, Ellenor
- Holmqvist, Marie
- Yohanoun, Peter
- Lindblad, Peter
- Stensjö, Karin
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