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Electron transfer b...
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Larsson, TedUppsala universitet,Fysikalisk-kemiska institutionen
(författare)
Electron transfer between cellobiose dehydrogenase and graphite electrodes
- Artikel/kapitelEngelska1996
Förlag, utgivningsår, omfång ...
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Elsevier,1996
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-150584
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-150584URI
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https://doi.org/10.1016/0003-2670(96)00136-5DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Electron transfer between the enzyme cellobiose dehydrogenase (CDH) and a flavin adenine dinucleotide (FAD) containing a catalytic active fragment of CDH (FAD-fragment) and a graphite electrode, respectively, was established. The current response in the presence of the enzyme substrate for graphite electrodes with CDH or the FAD-fragment adsorbed on the freshly polished graphite surface were compared with that of electrodes where CDH or the FAD-fragment were crosslinked in a redox polymer at the electrode surface. The initial slope, dj/d[S](S=0), where j is the current density and [S](S=0) the zero substrate concentration, was taken as a measure of the substrate response. For the electrodes with enzymes adsorbed directly on the surface, dj/d[S](S=0), was a factor of 3 lower than for electrodes prepared with the polymer mixture. The redox polymer based electrodes, with CDH and with FAD-fragment, both showed a high and close to equal substrate response. In contrast the surface adsorbed CDH gave a much higher substrate response than the FAD-fragment.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Elmgren, Maja,1964-Uppsala universitet,Fysikalisk-kemiska institutionen,Fysikalisk kemi(Swepub:uu)majaelmg
(författare)
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Lindquist, Sten-EricUppsala universitet,Fysikalisk-kemiska institutionen(Swepub:uu)stenlind
(författare)
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Tessema, Merid
(författare)
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Gorton, Lo
(författare)
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Henriksson, GunnarUppsala universitet,Biokemi
(författare)
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Uppsala universitetFysikalisk-kemiska institutionen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Analytica Chimica Acta: Elsevier331:3, s. 207-2150003-26701873-4324
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