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Type-specific detec...
Type-specific detection of high-risk human papillomavirus (HPV) in self-sampled cervicovaginal cells applied to FTA elute cartridge
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- Gustavsson, Inger, 1965- (author)
- Uppsala universitet,Genomik,Cervical cancer group
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- Sanner, Karin (author)
- Uppsala universitet,Obstetrik & gynekologi
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Lindell, Monica (author)
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- Strand, Anders (author)
- Uppsala universitet,Dermatologi och venereologi
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- Olovsson, Matts (author)
- Uppsala universitet,Obstetrik & gynekologi
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- Wikström, Ingrid (author)
- Uppsala universitet,Obstetrik & gynekologi
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- Wilander, Erik (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Gyllensten, Ulf (author)
- Uppsala universitet,Genomik
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(creator_code:org_t)
- Elsevier BV, 2011
- 2011
- English.
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In: Journal of Clinical Virology. - : Elsevier BV. - 1386-6532 .- 1873-5967. ; 51:4, s. 255-258
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- BackgroundMost procedures for self-sampling of cervical cells are based on liquid-based media for transportation and storage. An alternative is to use a solid support, such as dry filter paper media.ObjectivesTo evaluate if self-sampling of cervicovaginal fluid using a cytobrush (Viba-brush; Rovers Medical Devices B.V., Oss, The Netherlands) and a solid support such as the Whatman Indicating FTA® Elute cartridge (GE Healthcare, United Kingdom) can be used for reliable typing of human papillomavirus (HPV), as compared to cervical samples obtained by a physician using a cytobrush and the indicating FTA® Elute Micro card and biopsy analysis.Study designA total of 50 women with a previous high-risk (HR) HPV positive test were invited to perform self-sampling using the Viba-brush and the FTA cartridge and thereafter a physician obtained a cervical sample using the cytobrush and a FTA card, together with a cervical biopsy for histology and HPV typing. Detection of HR-HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59 was performed using three multiplex real-time polymerase chain reaction (PCR) assays.ResultAll samples contained sufficient amounts of genomic DNA and the self-samples yielded on average 3.5 times more DNA than those obtained by the physician. All women that were positive for HR-HPV in the biopsy sample also typed positive both by self-sampling and physician-obtained sampling. For women with a histological diagnosis of cervical intraepithelial neoplasia grades 2–3 (CIN 2–3) all three HPV samples showed 100% concordance. A higher number of women were HPV positive by self-sampling than by physician-obtained sampling or by biopsy analysis.ConclusionThe Viba-brush and the FTA cartridge are suitable for self-sampling of vaginal cells and subsequent HR-HPV typing.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Self-sampling
- FTA cartridge
- Cervical carcinoma
- Human papillomavirus (HPV)
- Real-time polymerase chain reaction (PCR)
- Clinical virology
- Klinisk virologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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