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Effects of affinity...
Effects of affinity on binding of HER2-targeting Affibody molecules : Model experiments in breast cancer spheroids
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- Qvarnström, O. Fredrik (author)
- Uppsala universitet,Enheten för onkologi
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- Simonsson, Martin (author)
- Uppsala universitet,Enheten för onkologi
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- Carlsson, Jörgen (author)
- Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap
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- Tran, Thuy A. (author)
- Lund University,Lunds universitet,Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap,Lund University Bioimaging Center,Medicinska fakulteten,Faculty of Medicine
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(creator_code:org_t)
- 2011-05-16
- 2011
- English.
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In: International Journal of Oncology. - : Spandidos Publications. - 1019-6439 .- 1791-2423. ; 39:2, s. 353-359
- Related links:
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https://www.spandido...
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://lup.lub.lu.s...
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Abstract
Subject headings
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- Binding of a targeting agent in tumor tissue is influenced by many factors such as molecular weight, charge and affinity of the targeting agent and vascularization of the tumor. In this study, we analyzed tumor cell binding of three HER2-specific and radiolabeled Affibody molecules with different affinities. The Affibody molecules had affinities in the range of 0.12-3.8 nM. Cellular binding was analyzed, after 2 h of incubation, in tumor spheroids composed of BT474 breast cancer cells, which highly express HER2. Binding was, due to the binding-site barrier,limited to the outer 15 +/- 5 mu m rim of the spheroids, independent of affinity when the concentration of the substances was low. When the concentration was high, the binding site barrier was overcome and the binding occurred approximately 35 +/- 5 mu m into the spheroids for the two high affinity substances and 50 +/- 5 mu m for the low affinity substance. The lower affinity might allow for penetration into deeper regions due to less firm binding. We conclude that there is a binding site barrier within tumor spheroids which can be overcome by increased concentration of substance and modified by affinity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Affibody
- affinity
- binding
- breast cancer cells
- BT474
- cell spheroids
- compound development
- HER2
- penetration
- radionuclides
- binding-site barrier
- MEDICINE
- MEDICIN
- Affibody
- affinity
- binding
- breast cancer cells
- BT474
- cell
- spheroids
- compound development
- HER2
- penetration
- radionuclides
- binding-site barrier
Publication and Content Type
- ref (subject category)
- art (subject category)
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