High-capacity Conductive Nanocellulose Paper Sheets for Electrochemically Controlled Extraction of DNA Oligomers

Razaq, AamirUppsala universitet,Nanoteknologi och funktionella material (författare)

Highly porous polypyrrole (PPy)-nanocellulose paper sheets have been evaluated as inexpensive and disposable electrochemically controlled three-dimensional solid phase extraction materials. The composites, which had a total anion exchange capacity of about 1.1 mol kg(-1), were used for extraction and subsequent release of negatively charged fluorophore tagged DNA oligomers via galvanostatic oxidation and reduction of a 30-50 nm conformal PPy layer on the cellulose substrate. The ion exchange capacity, which was, at least, two orders of magnitude higher than those previously reached in electrochemically controlled extraction, originated from the high surface area (i.e. 80 m(2) g(-1)) of the porous composites and the thin PPy layer which ensured excellent access to the ion exchange material. This enabled the extractions to be carried out faster and with better control of the PPy charge than with previously employed approaches. Experiments in equimolar mixtures of (dT)(6), (dT)(20), and (dT)(40) DNA oligomers showed that all oligomers could be extracted, and that the smallest oligomer was preferentially released with an efficiency of up to 40% during the reduction of the PPy layer. These results indicate that the present material is very promising for the development of inexpensive and efficient electrochemically controlled ion-exchange membranes for batch-wise extraction of biomolecules.

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
NATURVETENSKAP Kemi Oorganisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Inorganic Chemistry hsv//eng
TEKNIK OCH TEKNOLOGIER Nanoteknik hsv//swe
ENGINEERING AND TECHNOLOGY Nano-technology hsv//eng
Inorganic chemistry
Oorganisk kemi
Analytical Chemistry
Analytisk kemi
Teknisk fysik med inriktning mot nanoteknologi och funktionella material
Engineering Science with specialization in Nanotechnology and Functional Materials
Kemi med inriktning mot oorganisk kemi
Chemistry with specialization in Inorganic Chemistry
TECHNOLOGY

Gustav, NyströmUppsala universitet,Nanoteknologi och funktionella material(Swepub:uu)gusny218 (författare)
Maria, StrömmeUppsala universitet,Nanoteknologi och funktionella material(Swepub:uu)mst26208 (författare)
Mihranyan, AlbertUppsala universitet,Nanoteknologi och funktionella material(Swepub:uu)almih677 (författare)
Nyholm, LeifUppsala universitet,Oorganisk kemi(Swepub:uu)lny18455 (författare)
Uppsala universitetNanoteknologi och funktionella material (creator_code:org_t)

Av författaren/redakt...: Razaq, Aamir; Gustav, Nyström; Maria, Strömme; Mihranyan, Alber ...; Nyholm, Leif

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