Sökning: onr:"swepub:oai:DiVA.org:uu-159062" >
Distribution of ado...
Distribution of adoptively transferred porcine T-lymphoblasts tracked by (18)F-2-fluoro-2-deoxy-D-glucose and position emission tomography
-
- Eriksson, Olof (författare)
- Uppsala universitet,Enheten för onkologi
-
- Sadeghi, Arian (författare)
- Uppsala universitet,Klinisk immunologi
-
- Carlsson, Björn (författare)
- Uppsala universitet,Klinisk immunologi
-
visa fler...
-
- Eich, Torsten (författare)
- Uppsala universitet,Klinisk immunologi
-
- Lundgren, Torbjörn (författare)
- Karolinska Institutet
-
- Nilsson, Bo (författare)
- Uppsala universitet,Klinisk immunologi
-
- Tötterman, Thomas (författare)
- Uppsala universitet,Klinisk immunologi
-
- Korsgren, Olle (författare)
- Uppsala universitet,Klinisk immunologi
-
- Sundin, Anders (författare)
- Karolinska Institutet,Uppsala universitet,Enheten för radiologi
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2011
- 2011
- Engelska.
-
Ingår i: Nuclear Medicine and Biology. - : Elsevier BV. - 0969-8051 .- 1872-9614. ; 38:6, s. 827-833
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Introduction: Autologous or allogeneic transfer of tumor-infiltrating T-Iymphocytes is a promising treatment for metastatic cancers, but a major concern is the difficulty in evaluating cell trafficking and distribution in adoptive cell therapy. This study presents a method of tracking transfusion of T-Iymphoblasts in a porcine model by (18)F-2-fluoro-2-deoxy-D-glucose ([(18)F]FDG) and positron emission tomography. Methods: T-Iymphoblasts were labeled with the positron-emitting tracer [(18)F]FDG through incubation. The T-Iymphoblasts were administered into the bloodstream, and the distribution was followed by positron emission tomography for 120 min. The cells were administered either intravenously into the internal jugular vein (n=5) or intraarterially into the ascending aorta (n=1). Two of the pigs given intravenous administration were pretreated with low-molecular-weight dextran sulphate. Results: The cellular kinetics and distribution were readily quantifiable for up to 120 min. High (78.6% of the administered cells) heterogeneous pulmonary uptake was found after completed intravenous transfusion. The pulmonary uptake was decreased either by preineubating and coadministrating the T-Iymphoblasts with low-molecular-weight dextran sulphate or by administrating them intraarterially. Conclusions: The present work shows the feasibility of quantitatively monitoring and evaluating cell trafficking and distribution following administration of [(18)F]FDG-labeled T-Iymphoblasts. The protocol can potentially be transferred to the clinical setting with few modifications.
Nyckelord
- Adoptive transfer
- Cell trafficking
- Positron emission tomography
- T-Iymphoblasts
- [(18)F]FDG
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas