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CpG-A and CpG-B oli...
CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro.
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Rothenfusser, Simon (författare)
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Hornung, Veit (författare)
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Ayyoub, Maha (författare)
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Britsch, Stefanie (författare)
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Towarowski, Andreas (författare)
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Krug, Anne (författare)
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Sarris, Anja (författare)
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- Lubenow, Norbert (författare)
- Department of Transfusion Medicine, Greifswald University, Germany
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Speiser, Daniel (författare)
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Endres, Stefan (författare)
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Hartmann, Gunther (författare)
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(creator_code:org_t)
- American Society of Hematology, 2004
- 2004
- Engelska.
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Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 103:6, s. 2162-9
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Two distinct types of CpG oligodeoxynucleotide (ODN) have been identified that differ in their capacity to stimulate antigen-presenting cells: CpG-A induces high amounts of interferon-alpha (IFN-alpha) and IFN-beta in plasmacytoid dendritic cells (PDCs), whereas CpG-B induces PDC maturation and is a potent activator of B cells but stimulates only small amounts of IFN-alpha and IFN-beta. Here we examined the ability of these CpG ODNs to enhance peptide-specific CD8+ T-cell responses in human peripheral blood mononuclear cells (PBMCs). The frequency of influenza matrix-specific "memory" CD8+ T cells was increased by both types of CpG ODN, whereas the frequency of Melan-A specific "naive" CD8+ T cells increased on stimulation with CpG-B but not with CpG-A. The presence of PDCs in PBMCs was required for this CpG ODN-mediated effect. The expanded cells were cytotoxic and produced IFN- on peptide restimulation. Soluble factors induced by CpG-A but not CpG-B increased the granzyme-B content and cytotoxicity of established CD8+ T-cell clones, each of which was IFN-alpha/-beta dependent. In conclusion, CpG-B seems to be superior for priming CD8+ T-cell responses, and CpG-A selectively enhances memory CD8+ T-cell responses and induces cytotoxicity. These results demonstrate distinct functional properties of CpG-A and CpG-B with regard to CD8 T cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
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Blood
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Rothenfusser, Si ...
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Hornung, Veit
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Ayyoub, Maha
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Britsch, Stefani ...
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Towarowski, Andr ...
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Krug, Anne
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Sarris, Anja
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Lubenow, Norbert
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Speiser, Daniel
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Endres, Stefan
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Hartmann, Gunthe ...
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