Sökning: onr:"swepub:oai:DiVA.org:uu-173431" >
A T-Shaped Amphiphi...
A T-Shaped Amphiphilic Molecule Forms Closed Vesicles in Water and Bicelles in Mixtures with a Membrane Lipid
-
- Scholtysek, Peggy (författare)
- Institute of Chemistry - Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Germany
-
- Achilles, Anja (författare)
- Institute of Physics - NMR, Martin-Luther-University Halle-Wittenberg, Germany
-
- Hoffmann, Claudia-Viktoria (författare)
- Institute of Chemistry - Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Germany
-
visa fler...
-
- Lechner, Bob-Dan (författare)
- Institute of Chemistry - Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Germany
-
- Meister, Annette (författare)
- ZIK HALOmem, Martin-Luther-University Halle-Wittenberg, Germany
-
- Tschierske, Carsten (författare)
- Institute of Chemistry - Organic Chemistry, Martin-Luther-University Halle-Wittenberg, Germany
-
- Saalwächter, Kay (författare)
- Institute of Physics - NMR, Martin-Luther-University Halle-Wittenberg, Germany
-
- Edwards, Katarina (författare)
- Uppsala universitet,Analytisk kemi
-
- Blume, Alfred (författare)
- Institute of Chemistry - Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Germany
-
visa färre...
-
(creator_code:org_t)
- 2012-04-17
- 2012
- Engelska.
-
Ingår i: Journal of Physical Chemistry B. - : American Chemical Society (ACS). - 1520-6106 .- 1520-5207. ; 116:16, s. 4871-4878
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The T-shaped amphiphilic molecule A6/6 forms a columnar hexagonal liquid-crystalline phase between the crystalline and the isotropic liquid when studied in bulk (Chen et al., 2005). Because of the hydrophilic and flexible oligo(oxyethylene) side chain terminated by a 1-acylamino-1-deoxy-d-sorbitol moiety attached to a rigid terphenyl core with terminal hexyloxy alkyl chains, it was expected that also formation of lyotropic phases could be possible. We therefore studied the behavior of A6/6 in water and also in mixtures with bilayer-forming phospholipids, such as dipalmitoyl-phosphatidylcholine (DPPC), using differential scanning calorimetry (DSC), transmission electron microscopy (TEM), cryo-transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), and solid-state nuclear magnetic resonance (ssNMR). DSC showed for the pure A6/6 suspended in water a phase transition at ca. 23 °C. TEM and cryo-TEM showed vesicular as well as layered structures for pure A6/6 in water below and above this phase transition. By atomic force microscopy (AFM), the thickness of the layer was found to be 5?6 nm. This leads to a model for a bilayer formed by A6/6 with the laterally attached polar side chains shielding the hydrophobic layer built up by the terphenyl core with the terminal alkyl chains of the molecules. For DPPC:A6/6 mixtures (10:1), the DSC curves indicated a stabilization of the lamellar gel phase of DPPC. Negative staining TEM and cryo-TEM images showed planar bilayers with hexagonal morphology and diameters between 50 and 200 nm. The hydrodynamic radius of these aggregates in water, investigated by dynamic light scattering (DLS) as a function of time and temperature, did not change indicating a very stable aggregate structure. The findings lead to the proposition of a new bicellar structure formed by A6/6 with DPPC. In this model, the bilayer edges are covered by the T-shaped amphiphilic molecules preventing very effectively the aggregation to larger structures.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas