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A Potential Role fo...
A Potential Role for Chondroitin Sulfate/Dermatan Sulfate in Arm Regeneration in Amphiura filiformis.
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- Ramachandra, Rashmi (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Dorothe Spillmann
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- Namburi, Ramesh Babu (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Dorothe Spillmann
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- Dupont, Sam (författare)
- Department of Biological and Environmental Sciences, University of Gothenburg
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- Ortega-Martínez, Olga, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap, Kristineberg,Sven Lovén centrum för marina vetenskaper,Department of Biological and Environmental Sciences, Kristineberg,The Sven Lovén Centre for Marine Sciences
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- Thorndyke, Michael (författare)
- Department of Biological and Environmental Sciences, University of Gothenburg
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- Lindahl, Ulf (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Spillmann, Dorothe (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Dorothe Spillmann
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- Dupont, Samuel, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Sven Lovén centrum för marina vetenskaper,Institutionen för biologi och miljövetenskap, Kristineberg,The Sven Lovén Centre for Marine Sciences,Department of Biological and Environmental Sciences, Kristineberg
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van Kuppevelt, TH (författare)
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(creator_code:org_t)
- 2017-01-27
- 2017
- Engelska.
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Ingår i: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 27:5, s. 438-449
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Glycosaminoglycans (GAGs), such as chondroitin sulfate (CS) and dermatan sulfate (DS) from various vertebrate and invertebrate sources are known to be involved in diverse cellular mechanisms during repair and regenerative processes. Recently, we have identified CS/DS as the major GAG in the brittlestar Amphiura filiformis, with high proportions of di- and tri-O-sulfated disaccharide units. As this echinoderm is known for its exceptional regeneration capacity, we aimed to explore the role of these GAG chains during A. filiformis arm regeneration. Analysis of CS/DS chains during the regeneration process revealed an increase in the proportion of the tri-O-sulfated disaccharides. Conversely, treatment of A. filiformis with sodium chlorate, a potent inhibitor of sulfation reactions in GAG biosynthesis, resulted in a significant reduction in arm growth rates with total inhibition at concentrations higher than 5 mM. Differentiation was less impacted by sodium chlorate exposure or even slightly increased at 1-2 mM. Based on the structural changes observed during arm regeneration we identified chondroitin synthase, chondroitin-4-O-sulfotransferase 2 and dermatan-4-O-sulfotransferase as candidate genes and sought to correlate their expression with the expression of the A. filiformis orthologue of bone morphogenetic factors, AfBMP2/4. Quantitative amplification by real-time PCR indicated increased expression of chondroitin synthase and chondroitin-4-O-sulfotransferase 2, with a corresponding increase in AfBMP2/4 during regeneration relative to nonregenerating controls. Our findings suggest that proper sulfation of GAGs is important for A. filiformis arm regeneration and that these molecules may participate in mechanisms controlling cell proliferation.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Echinoderm
- Brittlestars
- Chondroitin sulfate
- Dermatan sulfate
- Sodium chlorate
- Amphiura filiformis
- brittlestar
- chondroitin sulfate
- dermatan sulfate
- regeneration
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Ramachandra, Ras ...
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Namburi, Ramesh ...
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Dupont, Sam
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Ortega-Martínez, ...
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Thorndyke, Micha ...
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Lindahl, Ulf
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Spillmann, Dorot ...
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Dupont, Samuel, ...
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van Kuppevelt, T ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Glycobiology
- Av lärosätet
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Uppsala universitet
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Göteborgs universitet