Sökning: onr:"swepub:oai:DiVA.org:uu-188407" >
Oxygen-charged HTK-...
Oxygen-charged HTK-F6H8 emulsion reduces ischemia : reperfusion injury in kidneys from brain-dead pigs
-
- Asif, Sana (författare)
- Uppsala universitet,Klinisk immunologi,korsgren
-
- Sedigh, Amir (författare)
- Uppsala universitet,Transplantationskirurgi
-
- Nordström, Johan (författare)
- Karolinska Institutet
-
visa fler...
-
- Brandhorst, Heide (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
-
- Jorns, Carl (författare)
- Karolinska Institutet
-
- Lorant, Tomas (författare)
- Uppsala universitet,Transplantationskirurgi
-
- Larsson, Erik (författare)
- Uppsala universitet,Molekylär och morfologisk patologi,alafuzoff
-
- Magnusson, Peetra U. (författare)
- Uppsala universitet,Klinisk immunologi,magnusson
-
- Nowak, Greg (författare)
- Karolinska Institutet
-
- Theisinger, Sonja (författare)
- Novaliq GmbH, Heidelberg, Germany
-
- Hoeger, Simone (författare)
- Department of Nephrology, Endocrinology and Rheumatology, University Medical Center Mannheim, Mannheim, Germany
-
- Wennberg, Lars (författare)
- Karolinska Institutet
-
- Korsgren, Olle (författare)
- Uppsala universitet,Klinisk immunologi,korsgren
-
- Brandhorst, Daniel (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- Engelska.
-
Ingår i: Journal of Surgical Research. - : Elsevier BV. - 0022-4804 .- 1095-8673. ; 178:2, s. 959-967
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background:Prolonged cold ischemia is frequently associated with a greater risk of delayed graft function and enhanced graft failure. We hypothesized that media, combining a high oxygen-dissolving capacity with specific qualities of organ preservation solutions, would be more efficient in reducing immediate ischemia-reperfusion injury from organs stored long term compared with standard preservation media.Methods:Kidneys retrieved from brain-dead pigs were flushed using either cold histidine-tryptophan-ketoglutarate (HTK) or oxygen-precharged emulsion composed of 75% HTK and 25% perfluorohexyloctane. After 18 h of cold ischemia the kidneys were transplanted into allogeneic recipients and assessed for adenosine triphosphate content, morphology, and expression of genes related to hypoxia, environmental stress, inflammation, and apoptosis.Results:Compared with HTK-flushed kidneys, organs preserved using oxygen-precharged HTK-perfluorohexyloctane emulsion had increased elevated adenosine triphosphate content and a significantly lower gene expression of hypoxia inducible factor-1 alpha, vascular endothelial growth factor, interleukin-1 alpha, tumor necrosis factor-alpha, interferon-alpha, JNK-1, p38, cytochrome-c, Bax, caspase-8, and caspase-3 at all time points assessed. In contrast, the mRNA expression of Bcl-2 was significantly increased.Conclusions:The present study has demonstrated that in brain-dead pigs the perfusion of kidneys with oxygen-precharged HTK-perfluorohexyloctane emulsion results in significantly reduced inflammation, hypoxic injury, and apoptosis and cellular integrity and energy content are well maintained. Histologic examination revealed less tubular, vascular, and glomerular changes in the emulsion-perfused tissue compared with the HTK-perfused counterparts. The concept of perfusing organs with oxygen-precharged emulsion based on organ preservation media represents an efficient alternative for improved organ preservation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Brain death
- Emulsion
- Kidney transplantation
- Organ preservation
- Oxygen carrier
- Pig
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Asif, Sana
-
Sedigh, Amir
-
Nordström, Johan
-
Brandhorst, Heid ...
-
Jorns, Carl
-
Lorant, Tomas
-
visa fler...
-
Larsson, Erik
-
Magnusson, Peetr ...
-
Nowak, Greg
-
Theisinger, Sonj ...
-
Hoeger, Simone
-
Wennberg, Lars
-
Korsgren, Olle
-
Brandhorst, Dani ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Kirurgi
- Artiklar i publikationen
-
Journal of Surgi ...
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet