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Plasmodium falcipar...
Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment in Tanzanian children with acute uncomplicated malaria
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- Carlsson, Anja M (författare)
- Karolinska Institutet
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Ngasala, Billy E (författare)
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Dahlström, Sabina (författare)
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Membi, Christopher (författare)
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Veiga, Isabel M (författare)
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- Rombo, Lars (författare)
- Karolinska Institutet,Uppsala universitet,Centrum för klinisk forskning i Sörmland (CKFD)
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Abdulla, Salim (författare)
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Premji, Zul (författare)
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- Gil, J Pedro (författare)
- Karolinska Institutet
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- Björkman, Anders (författare)
- Karolinska Institutet
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Mårtensson, Andreas (författare)
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(creator_code:org_t)
- 2011-12-20
- 2011
- Engelska.
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Ingår i: Malaria Journal. - : Springer Science and Business Media LLC. - 1475-2875. ; 10, s. 380-
- Relaterad länk:
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https://malariajourn...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
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- BACKGROUND:This study aimed to explore Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment with artemisinin-based combination therapy in children with clinical malaria in a high transmission area in Africa.METHODS:A total of 50 children aged 1-10 years with acute uncomplicated P. falciparum malaria in Bagamoyo District, Tanzania, were enrolled. Participants were hospitalized and received supervised standard treatment with artemether-lumefantrine according to body weight in six doses over 3 days. Blood samples were collected 11 times, i.e. at time of diagnosis (-2 h) and 0, 2, 4, 8, 16, 24, 36, 48, 60 and 72 h after initiation of treatment. Parasite population dynamics were assessed using nested polymerase chain reaction (PCR)-genotyping of merozoite surface protein (msp) 1 and 2.RESULTS:PCR-analyses from nine sequential blood samples collected after initiation of treatment identified 20 and 21 additional genotypes in 15/50 (30%) and 14/50 (28%) children with msp1 and msp2, respectively, non-detectable in the pre-treatment samples (-2 and 0 h combined). Some 15/20 (75%) and 14/21 (67%) of these genotypes were identified within 24 h, whereas 17/20 (85%) and 19/21 (90%) within 48 h for msp1 and msp2, respectively. The genotype profile was diverse, and varied considerably over time both within and between patients, molecular markers and their respective families.CONCLUSION:PCR analyses from multiple blood samples collected during the early treatment phase revealed a complex picture of parasite sub-populations. This underlines the importance of interpreting PCR-outcomes with caution and suggests that the present use of PCR-adjustment from paired blood samples in anti-malarial drug trials may overestimate assessment of drug efficacy in high transmission areas in Africa.The study is registered at http://www.clinicaltrials.gov with identifier NCT00336375.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Carlsson, Anja M
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Ngasala, Billy E
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Dahlström, Sabin ...
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Membi, Christoph ...
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Veiga, Isabel M
-
Rombo, Lars
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visa fler...
-
Abdulla, Salim
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Premji, Zul
-
Gil, J Pedro
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Björkman, Anders
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Mårtensson, Andr ...
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visa färre...
- Artiklar i publikationen
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Malaria Journal
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet