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Plasmodium falcipar...
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Carlsson, Anja MKarolinska Institutet
(författare)
Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment in Tanzanian children with acute uncomplicated malaria
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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2011-12-20
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Springer Science and Business Media LLC,2011
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-197823
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-197823URI
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https://doi.org/10.1186/1475-2875-10-380DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:124092580URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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BACKGROUND:This study aimed to explore Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment with artemisinin-based combination therapy in children with clinical malaria in a high transmission area in Africa.METHODS:A total of 50 children aged 1-10 years with acute uncomplicated P. falciparum malaria in Bagamoyo District, Tanzania, were enrolled. Participants were hospitalized and received supervised standard treatment with artemether-lumefantrine according to body weight in six doses over 3 days. Blood samples were collected 11 times, i.e. at time of diagnosis (-2 h) and 0, 2, 4, 8, 16, 24, 36, 48, 60 and 72 h after initiation of treatment. Parasite population dynamics were assessed using nested polymerase chain reaction (PCR)-genotyping of merozoite surface protein (msp) 1 and 2.RESULTS:PCR-analyses from nine sequential blood samples collected after initiation of treatment identified 20 and 21 additional genotypes in 15/50 (30%) and 14/50 (28%) children with msp1 and msp2, respectively, non-detectable in the pre-treatment samples (-2 and 0 h combined). Some 15/20 (75%) and 14/21 (67%) of these genotypes were identified within 24 h, whereas 17/20 (85%) and 19/21 (90%) within 48 h for msp1 and msp2, respectively. The genotype profile was diverse, and varied considerably over time both within and between patients, molecular markers and their respective families.CONCLUSION:PCR analyses from multiple blood samples collected during the early treatment phase revealed a complex picture of parasite sub-populations. This underlines the importance of interpreting PCR-outcomes with caution and suggests that the present use of PCR-adjustment from paired blood samples in anti-malarial drug trials may overestimate assessment of drug efficacy in high transmission areas in Africa.The study is registered at http://www.clinicaltrials.gov with identifier NCT00336375.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ngasala, Billy E
(författare)
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Dahlström, Sabina
(författare)
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Membi, Christopher
(författare)
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Veiga, Isabel M
(författare)
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Rombo, LarsKarolinska Institutet,Uppsala universitet,Centrum för klinisk forskning i Sörmland (CKFD)(Swepub:uu)larra728
(författare)
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Abdulla, Salim
(författare)
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Premji, Zul
(författare)
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Gil, J PedroKarolinska Institutet
(författare)
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Björkman, AndersKarolinska Institutet
(författare)
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Mårtensson, Andreas
(författare)
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Karolinska InstitutetCentrum för klinisk forskning i Sörmland (CKFD)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Malaria Journal: Springer Science and Business Media LLC10, s. 380-1475-2875
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Carlsson, Anja M
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Ngasala, Billy E
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Dahlström, Sabin ...
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Membi, Christoph ...
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Veiga, Isabel M
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Rombo, Lars
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visa fler...
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Abdulla, Salim
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Premji, Zul
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Gil, J Pedro
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Björkman, Anders
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Mårtensson, Andr ...
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- Artiklar i publikationen
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Malaria Journal
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet