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alpha B-crystallin/...
alpha B-crystallin/HspB5 regulates endothelial-leukocyte interactions by enhancing NF-kappa B-induced up-regulation of adhesion molecules ICAM-1, VCAM-1 and E-selectin
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- Dieterich, Lothar C. (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,The Rudbeck Laboratory
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- Huang, Hua (författare)
- Uppsala universitet,Cancer och vaskulärbiologi,The Rudbeck Laboratory
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- Massena, Sara (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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Golenhofen, Nikola (författare)
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- Phillipson, Mia (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Dimberg, Anna (författare)
- Uppsala universitet,Cancer och vaskulärbiologi,The Rudbeck Laboratory
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(creator_code:org_t)
- 2013-08-09
- 2013
- Engelska.
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Ingår i: Angiogenesis. - : Springer Science and Business Media LLC. - 0969-6970 .- 1573-7209. ; 16:4, s. 975-983
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- alpha B-crystallin is a small heat shock protein, which has pro-angiogenic properties by increasing survival of endothelial cells and secretion of vascular endothelial growth factor A. Here we demonstrate an additional role of alpha B-crystallin in regulating vascular function, through enhancing tumor necrosis factor alpha (TNF-alpha) induced expression of endothelial adhesion molecules involved in leukocyte recruitment. Ectopic expression of alpha B-crystallin in endothelial cells increases the level of E-selectin expression in response to TNF-alpha, and enhances leukocyte-endothelial interaction in vitro. Conversely, TNF-alpha-induced expression of intercellular adhesion molecule 1, vascular cell adhesion molecule 1 and E-selectin is markedly inhibited in endothelial cells isolated from alpha B-crystallin-deficient mice. This is associated with elevated levels of I kappa B in alpha B-crystallin deficient cells and incomplete degradation upon TNF-alpha stimulation. Consistent with this, endothelial adhesion molecule expression is reduced in inflamed vessels of alpha B-crystallin deficient mice, and leukocyte rolling velocity is increased. Our data identify alpha B-crystallin as a new regulator of leukocyte recruitment, by enhancing pro-inflammatory nuclear factor kappa B-signaling and endothelial adhesion molecule expression during endothelial activation.
Nyckelord
- alpha B-crystallin
- Chaperone
- ICAM-1
- VCAM-1
- E-selectin
- NF-kappa B
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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