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Interaction between...
Interaction between the GROWTH-REGULATING FACTOR and KNOTTED1-LIKE HOMEOBOX Families of Transcription Factors1[W]
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Kuijt, Suzanne J. H. (författare)
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Greco, Raffaella (författare)
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Agalou, Adamantia (författare)
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Shao, Jingxia (författare)
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't Hoen, Corine C. J. (författare)
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- Övernas, Elin (författare)
- Uppsala universitet,Fysiologisk botanik
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Osnato, Michela (författare)
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Curiale, Serena (författare)
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Meynard, Donaldo (författare)
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van Gulik, Robert (författare)
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Maraschin, Simone de Faria (författare)
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Atallah, Mirna (författare)
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de Kam, Rolf J. (författare)
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Lamers, Gerda E. M. (författare)
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Guiderdoni, Emmanuel (författare)
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Rossini, Laura (författare)
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Meijer, Annemarie H. (författare)
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Ouwerkerk, Pieter B. F. (författare)
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(creator_code:org_t)
- 2014-02-14
- 2014
- Engelska.
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 164:4, s. 1952-1966
- Relaterad länk:
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http://www.plantphys...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- KNOTTED1-LIKE HOMEOBOX (KNOX) genes are important regulators of meristem function, and a complex network of transcription factors ensures tight control of their expression. Here, we show that members of the GROWTH-REGULATING FACTOR (GRF) family act as players in this network. A yeast (Saccharomyces cerevisiae) one-hybrid screen with the upstream sequence of the KNOX gene Oskn2 from rice (Oryza sativa) resulted in isolation of OsGRF3 and OsGRF10. Specific binding to a region in the untranslated leader sequence of Oskn2 was confirmed by yeast and in vitro binding assays. ProOskn2: beta-glucuronidase reporter expression was down-regulated by OsGRF3 and OsGRF10 in vivo, suggesting that these proteins function as transcriptional repressors. Likewise, we found that the GRF protein BGRF1 from barley (Hordeum vulgare) could act as a repressor on an intron sequence in the KNOX gene Hooded/Barley Knotted3 (Bkn3) and that AtGRF4, AtGRF5, and AtGRF6 from Arabidopsis (Arabidopsis thaliana) could repress KNOTTED-LIKE FROM ARABIDOPSIS THALIANA2 (KNAT2) promoter activity. OsGRF overexpression phenotypes in rice were consistent with aberrant meristematic activity, showing reduced formation of tillers and internodes and extensive adventitious root/shoot formation on nodes. These effects were associated with down-regulation of endogenous Oskn2 expression by OsGRF3. Conversely, RNA interference silencing of OsGRF3, OsGRF4, and OsGRF5 resulted in dwarfism, delayed growth and inflorescence formation, and up-regulation of Oskn2. These data demonstrate conserved interactions between the GRF and KNOX families of transcription factors in both monocot and dicot plants.
Ämnesord
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
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- art (ämneskategori)
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- Av författaren/redakt...
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Kuijt, Suzanne J ...
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Greco, Raffaella
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Agalou, Adamanti ...
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Shao, Jingxia
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't Hoen, Corine ...
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Övernas, Elin
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visa fler...
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Osnato, Michela
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Curiale, Serena
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Meynard, Donaldo
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van Gulik, Rober ...
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Maraschin, Simon ...
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Atallah, Mirna
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de Kam, Rolf J.
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Lamers, Gerda E. ...
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Guiderdoni, Emma ...
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Rossini, Laura
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Meijer, Annemari ...
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Ouwerkerk, Piete ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Evolutionsbiolog ...
- Artiklar i publikationen
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Plant Physiology
- Av lärosätet
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Uppsala universitet