Sökning: onr:"swepub:oai:DiVA.org:uu-229724" > Characterization of...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 05240naa a2200565 4500 | |
001 | oai:DiVA.org:uu-229724 | |
003 | SwePub | |
008 | 140812s2014 | |||||||||||000 ||eng| | |
009 | oai:slubar.slu.se:64280 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2297242 URI |
024 | 7 | a https://doi.org/10.1128/CVI.00162-142 DOI |
024 | 7 | a https://res.slu.se/id/publ/642802 URI |
040 | a (SwePub)uud (SwePub)slu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Hägglund, Sarau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut0 (Swepub:slu)49235 |
245 | 1 0 | a Characterization of an Experimental Vaccine for Bovine Respiratory Syncytial Virus |
264 | 1 | c 2014 |
338 | a print2 rdacarrier | |
520 | a Bovine respiratory syncytial virus (BRSV) and human respiratory syncytial virus (HRSV) are major causes of respiratory disease in calves and children, respectively, and are priorities for vaccine development. We previously demonstrated that an experimental vaccine, BRSV-immunostimulating complex (ISCOM), is effective in calves with maternal antibodies. The present study focuses on the antigenic characterization of this vaccine for the design of new-generation subunit vaccines. The results of our study confirmed the presence of membrane glycoprotein (G), fusion glycoprotein (F), and nucleoprotein (N) proteins in the ISCOMs, and this knowledge was extended by the identification of matrix (M), M2-1, phosphoprotein (P), small hydrophobic protein (SH) and of cellular membrane proteins, such as the integrins alpha(V)beta(1), alpha(V)beta(3), and alpha(3)beta(1). The quantity of the major protein F was 4- to 5-fold greater than that of N (similar to 77 mu g versus similar to 17 mu g/calf dose), whereas G, M, M2-1, P, and SH were likely present in smaller amounts. The polymerase (L), M2-2, nonstructural 1 (NS1), and NS2 proteins were not detected, suggesting that they are not essential for protection. Sera from the BRSV-ISCOM-immunized calves contained high titers of IgG antibody specific for F, G, N, and SH. Antibody responses against M and P were not detected; however, this does not exclude their role in protective T-cell responses. The absence of immunopathological effects of the cellular proteins, such as integrins, needs to be further confirmed, and their possible contribution to adjuvant functions requires elucidation. This work suggests that a combination of several surface and internal proteins should be included in subunit RSV vaccines and identifies absent proteins as potential candidates for differentiating infected from vaccinated animals. | |
650 | 7 | a NATURVETENSKAPx Biologix Immunologi0 (SwePub)106052 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a LANTBRUKSVETENSKAPERx Veterinärmedicin0 (SwePub)4032 hsv//swe |
650 | 7 | a AGRICULTURAL SCIENCESx Veterinary Science0 (SwePub)4032 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng |
650 | 7 | a LANTBRUKSVETENSKAPERx Veterinärmedicinx Annan veterinärmedicin0 (SwePub)403042 hsv//swe |
650 | 7 | a AGRICULTURAL SCIENCESx Veterinary Sciencex Other Veterinary Science0 (SwePub)403042 hsv//eng |
700 | 1 | a Hu, Kefei4 aut |
700 | 1 | a Blodörn, Kristeru Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut0 (Swepub:slu)48694 |
700 | 1 | a Makabi-Panzu, Boby4 aut |
700 | 1 | a Gaillard, Anne-Laure4 aut |
700 | 1 | a Ellencrona, Karinu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut0 (Swepub:slu)52708 |
700 | 1 | a Chevret, Didier4 aut |
700 | 1 | a Hellman, Larsu Uppsala universitet,Kemisk biologi4 aut0 (Swepub:uu)larshell |
700 | 1 | a Bengtsson, Karin Lovgren4 aut |
700 | 1 | a Riffault, Sabine4 aut |
700 | 1 | a Taylor, Geraldine4 aut |
700 | 1 | a Valarcher, Jean-Francoisu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut0 (Swepub:slu)50389 |
700 | 1 | a Eleouet, Jean-Francois4 aut |
710 | 2 | a Sveriges lantbruksuniversitetb Institutionen för kliniska vetenskaper (KV)4 org |
710 | 2 | a Sveriges lantbruksuniversitet |
773 | 0 | t Clinical and Vaccine Immunologyg 21:7, s. 997-1004q 21:7<997-1004x 1556-6811x 1556-679X |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-229724 |
856 | 4 8 | u https://doi.org/10.1128/CVI.00162-14 |
856 | 4 8 | u https://res.slu.se/id/publ/64280 |
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