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Comparison of real-...
Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants
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- Storm, Martin (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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Advani, Abdolreza (författare)
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Pettersson, Monica (författare)
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Hallander, Hans O (författare)
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- Bondeson, Kåre (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
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Ingår i: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 65:1, s. 153-158
- Relaterad länk:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We describe two newly developed methods for rapid typing of the pertussis toxin subunit 1 gene (ptxS1). A real-time PCR assay based on hybridization probes and a Pyrosequencing assay were developed and the specificity, sensitivity, cost, hands-on time and post-assay data processing were compared to Sanger sequencing. Both methods enabled discrimination of all four allelic variants, correctly identified all ptxS1 alleles of 143 strains tested and proved suitable for large-scale screening of B. pertussis strains.
Nyckelord
- Alleles
- Bordetella pertussis/classification/*genetics
- DNA; Bacterial/chemistry/genetics
- Humans
- Pertussis Toxin/chemistry/*genetics
- Pertussis Vaccine/genetics
- Polymerase Chain Reaction
- Polymorphism; Single Nucleotide
- Sequence Analysis; DNA
- Whooping Cough/microbiology
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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