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Field deployment of...
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Morris, UlrikaKarolinska Institutet
(författare)
Field deployment of loop-mediated isothermal amplification for centralized mass-screening of asymptomatic malaria in Zanzibar : a pre-elimination setting
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
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2015-05-17
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Springer Science and Business Media LLC,2015
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-256149
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-256149URI
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https://doi.org/10.1186/s12936-015-0731-2DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:131308680URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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BACKGROUND: Molecular tools for detection of low-density asymptomatic Plasmodium infections are needed in malaria elimination efforts. This study reports results from the hitherto largest implementation of loop-mediated isothermal amplification (LAMP) for centralized mass screening of asymptomatic malaria in Zanzibar.METHODS: Healthy individuals present and willing to participate in randomly selected households in 60 villages throughout Zanzibar were screened for malaria by rapid diagnostic tests (RDT). In 50 % of the study households, participants were asked to provide 60 μL of finger-prick blood for additional LAMP screening. LAMP was conducted in two centralized laboratories in Zanzibar, by trained technicians with limited or no previous experience of molecular methods. The LAMP assay was performed with Loopamp(TM) MALARIA Pan/Pf Detection Kit (Eiken Chemical Company, Japan). Samples positive for Plasmodium genus (Pan)-LAMP were re-tested using Plasmodium falciparum-specific LAMP kits.RESULTS: Paired RDT and LAMP samples were available from 3983 individuals. The prevalence of asymptomatic malaria was 0.5 % (CI 95 % 0.1-0.8) and 1.6 % (CI 95 % 1.1-2.2) by RDT and Pan-LAMP, respectively. LAMP detected 3.4 (CI 95 % 2.2-5.2) times more Plasmodium positive samples than RDT. DNA contamination was experienced, but solved by repetitive decontamination of all equipment and reagents.CONCLUSIONS: LAMP is a simple and sensitive molecular tool, and has potential in active surveillance and mass-screening programmes for detection of low-density asymptomatic malaria in pre-elimination settings. However, in order to deploy LAMP more effectively in field settings, protocols may need to be adapted for processing larger numbers of samples. A higher throughput, affordable closed system would be ideal to avoid contamination.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Khamis, Mwinyi
(författare)
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Aydin-Schmidt, BeritKarolinska Institutet
(författare)
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Abass, Ali K
(författare)
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Msellem, Mwinyi I
(författare)
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Nassor, Majda H
(författare)
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González, Iveth J
(författare)
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Mårtensson, AndreasKarolinska Institutet,Uppsala universitet,Centrum för klinisk forskning i Sörmland (CKFD)(Swepub:uu)andma331
(författare)
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Ali, Abdullah S
(författare)
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Björkman, AndersKarolinska Institutet
(författare)
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Cook, Jackie
(författare)
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Karolinska InstitutetCentrum för klinisk forskning i Sörmland (CKFD)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Malaria Journal: Springer Science and Business Media LLC141475-2875
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Morris, Ulrika
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Khamis, Mwinyi
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Aydin-Schmidt, B ...
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Abass, Ali K
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Msellem, Mwinyi ...
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Nassor, Majda H
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visa fler...
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González, Iveth ...
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Mårtensson, Andr ...
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Ali, Abdullah S
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Björkman, Anders
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Cook, Jackie
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- MEDICIN OCH HÄLSOVETENSKAP
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och Klinisk medicin
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och Infektionsmedici ...
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Malaria Journal
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Uppsala universitet
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Karolinska Institutet