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Vascular repair uti...
Vascular repair utilising immobilised heparin conjugate for protection against early activation of inflammation and coagulation
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- Nordling, Sofia (författare)
- Uppsala universitet,Klinisk immunologi
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- Hong, Jaan (författare)
- Uppsala universitet,Klinisk immunologi
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- Fromell, Karin (författare)
- Uppsala universitet,Klinisk immunologi
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- Edin, Fredrik (författare)
- Uppsala universitet,Klinisk immunologi,Öron-, näs- och halssjukdomar
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- Brännström, Johan (författare)
- Uppsala universitet,Klinisk immunologi
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- Larsson, Rolf (författare)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin
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- Nilsson, Bo (författare)
- Uppsala universitet,Klinisk immunologi
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- Magnusson, Peetra U. (författare)
- Uppsala universitet,Klinisk immunologi
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(creator_code:org_t)
- 2015
- 2015
- Engelska.
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Ingår i: Thrombosis and Haemostasis. - 0340-6245 .- 2567-689X. ; 113:6, s. 1312-1322
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Ischaemia-reperfusion injury (IRI) poses a major challenge in many thrombotic conditions and in whole organ transplantation. Activation of the endothelial cells and shedding of the protective vascular glycocalyx during IRI increase the risk of innate immune activation, cell infiltration and severe thrombus formation, promoting damage to the tissue. Here, we present a novel one-step strategy to protect the vas, culature by immobilisation of a unique multi-arm heparin conjugate to the endothelium. Applying a new in vitro blood endothelial cell chamber model, the heparin conjugate was found to bind not only to primary human endothelial cells but also directly to the collagen to which the cells adhered. Incubation of hypoxic endothelial cells with freshly drawn human blood in the blood chambers elicited coagulation activation reflected by thrombin anti-thrombin formation and binding of platelets and neutrophils. Immobilisation of the heparin conjugate to the hypoxic endothelial cells created a protective coating, leading to a Significant reduction of the recruitment of blood cells and coagulation activation compared to untreated hypoxic endothelial cells. This novel approach of immobilising multi-arm heparin conjugates on the endothelial cells and collagen of the basement membrane ensures to protect the endothelium against IRI in thrombotic disorders and in transplantation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Nyckelord
- Blood coagulation
- endothelial cells
- heparin
- surface modification
- collagen
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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