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Prevention of HET-s...
Prevention of HET-s prion aggregation by ribosomal RNA
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Pang, Yanhong (författare)
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Macedo, Bruno (författare)
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Sanyal, Suparna (författare)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The role of nucleic acids in prion aggregation / disaggregation has remained unclear. Here, using HET-s prion from fungi Podospora anserina as a model system, we have studied the role of ribosomal RNA (rRNA) in its aggregation process in an unbiased manner. Our results show that rRNA, in particular domain V of rRNA from the large subunit of the ribosome, substantially prevents beta-amyloid aggregation of the HET-s prion in a concentration dependent manner. The sites of interaction of the HET-s prion on domain V rRNA have been identified, which overlap with the sites previously identified for the protein folding activity of the ribosome. This study provides a missing link for the role of rRNA based folding activity of the ribosome in the context of prion propagation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)